Invitrogen

Aminopeptidase P Monoclonal Antibody (JG12), eBioscience™

Product Image Gallery

Aminopeptidase P Antibody in Immunohistochemistry (IHC) — Fig. 4. Immunohistochemistry of pre- and post-transplantation kidneys. (A) JG12 + area/tubular field, (D) JG12 + area/glomerulus, (G) ED1 + cells/tubular field, (J) ED1 + cells/glomerulus and (K) glomerular area (scored on slides stained with JG12). (B,C,E,F,H,I) Representative fixed paraffin-embedded histology pre- (B,E,H) and post- (C,F,I) transplantation of an HD-CR combination (analogous to clinical transplantation with a living donor). HD, healthy donor; CD, donor with CKD (pre-Tx); HR, healthy recipient; CR, recipient with CKD (post-Tx). All n =6. Data are mean+-s.d.; two-way repeated measures ANOVA, Newman-Keuls post hoc test. * P <0.05 versus pre-transplantation. Interaction: D, P <0.05 for effect of graft on recipient; R, P <0.05 for effect of environment on graft. Pre-transplantation CD versus HD, P <0.05 for all variables (post hoc symbols not shown in graphs).

Product Details

BMS1104

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	Assay-Dependent	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Rat
Published species	Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	JG12
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20°C
RRID	AB_10597125

Product Specific Information

Description: Aminopeptidase P is a glycosyl-phosphatidylinositol (GPI) anchored membrane enzyme that is recognized by a monoclonal antibody designated JG12 that was raised against rat glomerular membrane protein fractions and labeled intensely microvessels of kidney and lung.

Applications Tested: Immunohistochemistry (Paraffin).

Target Information

This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effect of Calcitriol on the Renal Microvasculature Differentiation Disturbances Induced by AT₁ Blockade During Nephrogenesis in Rats.

Frontiers in medicine

Deluque AL,de Almeida LF,Francescato HDC,da Silva CGA,Costa RS,Antunes-Rodrigues J,Coimbra TM

BMS1104 was used in Immunohistochemistry to identify that calcitriol treatment can mitigate the alterations in renal microvasculature differentiation caused by AT1 blockade during lactation in rats.

Mon Sep 28 00:00:00 EDT 2020

Previous Exercise Effects in Cisplatin-Induced Renal Lesions in Rats.

Kidney & blood pressure research

Francescato HDC,Almeida LF,Reis NG,Faleiros CM,Papoti M,Costa RS,Coimbra TM

BMS1104 was used in Immunohistochemistry to investigate the effect of physical training on cisplatin induced acute kidney injury in rats.

Mon Oct 29 00:00:00 EDT 2018

Dissecting recipient from donor contribution in experimental kidney transplantation: focus on endothelial proliferation and inflammation.

Disease models & mechanisms

Papazova DA,Krebber MM,Oosterhuis NR,Gremmels H,van Zuilen AD,Joles JA,Verhaar MC

Published figure using Aminopeptidase P monoclonal antibody (Product # BMS1104) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2018

Exposure to placental ischemia impairs postpartum maternal renal and cardiac function in rats.

American journal of physiology. Regulatory, integrative and comparative physiology

Paauw ND,Joles JA,Spradley FT,Bakrania B,Zsengeller ZK,Franx A,Verhaar MC,Granger JP,Lely AT

BMS1104 was used in Immunohistochemistry to assess whether placental ischemia results in long-term effects on the maternal cardiovascular and renal systems using the reduced uterine perfusion pressure (RUPP) model for preeclampsia.

Mon May 01 00:00:00 EDT 2017

Distinct Endothelial Cell Responses in the Heart and Kidney Microvasculature Characterize the Progression of Heart Failure With Preserved Ejection Fraction in the Obese ZSF1 Rat With Cardiorenal Metabolic Syndrome.

Circulation. Heart failure

van Dijk CG,Oosterhuis NR,Xu YJ,Brandt M,Paulus WJ,van Heerebeek L,Duncker DJ,Verhaar MC,Fontoura D,Lourenço AP,Leite-Moreira AF,Falcão-Pires I,Joles JA,Cheng C

BMS1104 was used in Immunohistochemistry to evaluate the coronary and renal microvasculature during CRMS development in obese diabetic Zucker fatty/Spontaneously hypertensive heart failure F1 hybrid rats.

Fri Apr 01 00:00:00 EDT 2016

Mesenchymal stem cells (MSC) prevented the progression of renovascular hypertension, improved renal function and architecture.

PloS one

Oliveira-Sales EB,Maquigussa E,Semedo P,Pereira LG,Ferreira VM,Câmara NO,Bergamaschi CT,Campos RR,Boim MA

BMS1104 was used in Immunohistochemistry on paraffin embedded tissues to investigate alternative therapies for treatment of renovascular hypertension.

Fri Aug 08 00:00:00 EDT 2014

C-peptide preserves the renal microvascular architecture in the streptozotocin-induced diabetic rat.

Journal of diabetes and its complications

Flynn ER,Lee J,Hutchens ZM,Chade AR,Maric-Bilkan C

BMS1104 was used in Immunohistochemistry on paraffin embedded tissues to suggest that C-peptide is renoprotective by preserving the integrity of the renal microvasculature irrespective of glucose regulation.

Tue Jul 01 00:00:00 EDT 2014

Combination therapy with an angiotensin II receptor blocker and an HMG-CoA reductase inhibitor in experimental subtotal nephrectomy.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Álvarez-Prats A,Hernández-Perera O,Díaz-Herrera P,Ucero ÁC,Anabitarte-Prieto A,Losada-Cabrera A,Ortiz A,Rodríguez-Pérez JC

BMS1104 was used in Immunohistochemistry to assess the therapeutic potential of the use of statins in chronic kidney disease.

Sun Jul 01 00:00:00 EDT 2012

Microvascular disease precedes the decline in renal function in the streptozotocin-induced diabetic rat.

American journal of physiology. Renal physiology

Maric-Bilkan C,Flynn ER,Chade AR

BMS1104 was used in Immunohistochemistry to investigate whether changes in renal microvascular density correlate with and play a role in the progressive deterioration of renal function in diabetes.

Wed Feb 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Aminoacylproline aminopeptidase; cytoplasmic aminopeptidase P; Cytosolic aminopeptidase P; OTTHUMP00000020457; OTTHUMP00000058856; sAmp; Soluble aminopeptidase P; X-Pro aminopeptidase 1; X-Pro aminopeptidase 1, soluble; X-prolyl aminopeptidase 1, soluble; Xaa-Pro aminopeptidase 1; xpnpep1 {ECO:0000312|RGD:621274}

Gene Aliases: App; Xpnpep1

UniProt ID: (Rat) O54975

Entrez Gene ID: (Rat) 170751

Molecular Function: protease