|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic peptide corresponding to residues 1-16 of beta Amyloid.|
|Storage buffer||PBS, pH 7.2, with 50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-25493 detects beta Amyloid from human and mouse (weakly) samples.
MA1-25493 has been successfully used in Western blot and immunohistochemistry procedures. Western blot detects a band ~4-5 kDa.
The MA1-25493 immunogen is a synthetic peptide corresponding to residues 1-16 of beta Amyloid.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SCF TrCP1 activates and ubiquitylates TAp63gamma.
MA1-25493 was used in western blot to study the activation and ubiquitinylation of TAp63gamma by SCF TrCP1
|Gallegos JR,Litersky J,Lee H,Sun Y,Nakayama K,Nakayama K,Lu H||The Journal of biological chemistry (283:66)||2008|