Immunohistochemistry analysis of Androgen Receptor showing staining in the cytoplasm of formalin-fixed, paraffin-embedded human prostate tissue (B) and magnified section (C) compared with an isotype control (A). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2). Tissues were probed with an Androgen Receptor monoclonal antibody (Product # MA1-150) for 60 minutes at a dilution of 2ug/ml and detection was performed using an HRP-conjugated mouse anti-rat secondary antibody for 45 minutes followed by DAB staining.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Non-human primate, Mouse, Human|
|Host / Isotype||Rat / IgG|
|Immunogen||A fusion protein corresponding to residues 331-572 of human androgen receptor.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||2-4 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/mL|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-150 detects androgen receptor from human, Rhesus monkey, rat and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors.
MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining.
The MA1-150 immunogen is a fusion protein corresponding to residues 331-572 of human androgen receptor.
Reconstitute with PBS.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential regulation of gonadotropin-releasing hormone secretion and gene expression by androgen: membrane versus nuclear receptor activation.
MA1-150 was used in western blot to investigate the effect of androgen on gonadotropin-releasing hormone secretion and gene expression.
|Shakil T,Hoque AN,Husain M,Belsham DD||Molecular endocrinology (Baltimore, Md.) (16:2592)||2002|
Cyclin E as a coactivator of the androgen receptor.
MA1-150 was used in western blot to study the relationship between cyclin E ad androgen receptor.
|Yamamoto A,Hashimoto Y,Kohri K,Ogata E,Kato S,Ikeda K,Nakanishi M||The Journal of cell biology (150:873)||2000|
Expression of maspin in prostate cells is regulated by a positive ets element and a negative hormonal responsive element site recognized by androgen receptor.
MA1-150 was used in EMSA to study the transcriptional regulation of maspin in the prostate.
|Zhang M,Magit D,Sager R||Proceedings of the National Academy of Sciences of the United States of America (94:5673)||1997|
Human androgen receptor binding to the androgen response element of prostate specific antigen.
MA1-150 was used in EMSA to study the in vitro binding of the human androgen receptor to the putative androgen response element of prostate specific antigen
|Luke MC,Coffey DS||Journal of andrology (15:41)||1994|
Atypical androgen receptor in the human melanoma cell line IIB-MEL-J.
MA1-150 was used in immunohistochemistry to investigate the expression of androgen receptor in IIB-MEL-J cells
|Morvillo V,Luthy IA,Bravo AI,Capurro MI,Donaldson M,Quintans C,Calandra RS,Mordoh J||Pigment cell research (8:135)||1995|
Evidence for the presence of androgen receptors in the synovial tissue of rheumatoid arthritis patients and healthy controls.
MA1-150 was used in immunohistochemistry to investigate the expression of androgen receptor in the synovial tissue from rheumatoid arthritic patients
|Cutolo M,Accardo S,Villaggio B,Clerico P,Indiveri F,Carruba G,Fecarotta E,Castagnetta L||Arthritis and rheumatism (35:1007)||1992|
Immunohistochemistry and biochemistry in detection of androgen, progesterone, and estrogen receptors in benign and malignant human prostatic tissue.
MA1-150 was used in immunohistochemistry to investigate the distribution of AR, ER and PR in human prostatic tumor cell lines
|Brolin J,Skoog L,Ekman P||The Prostate (20:281)||1992|
Prostate androgen receptor: immunohistological localization and mRNA characterization.
MA1-150 was used in immunohistochemistry to investigate the distribution of androgen receptor in vivo
|Chang C,Chodak G,Sarac E,Takeda H,Liao S||Journal of steroid biochemistry (34:311)||1990|
|Non-human primate||3.7 ug/ml||
Androgen receptor and 5 alpha-reductase activity in the ductuli efferentes and epididymis of adult rhesus macaques.
MA1-150 was used in immunocytochemistry to study the immunocytochemical distribution of AR in comparison with biochemical quantification of AR and 5 alpha-reductase activity in the ductuli efferentes and epididymides of adult male rhesus monkeys
|Roselli CE,West NB,Brenner RM||Biology of reproduction (44:739)||1991|