|Gel Shift (GS)||Assay dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||2-4 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/mL|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:50-1:500|
|Western Blot (WB)||See 2 publications below|
|Gel Shift (GS)||See 2 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Non-human primate , Mouse Human|
|Host / Isotype||Rat / IgG|
|Immunogen||A fusion protein corresponding to residues 331-572 of human androgen receptor.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-150 detects androgen receptor from human, Rhesus monkey, rat and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors.
MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining.
The MA1-150 immunogen is a fusion protein corresponding to residues 331-572 of human androgen receptor.
Reconstitute with PBS.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ANDR; androgen nuclear receptor variant 2; Androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4
Gene Aliases: AIS; Andr; AR; AR8; AW320017; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
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