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|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length, human Angiopoietin 2.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20178 detects Angiopoietin 2 in human samples.
PA1-20178 has been successfully used in ELISA and Western blot procedures.
The PA1-20178 immunogen is recombinant, full length, human Angiopoietin 2.
Angiopoietin 2 and angiopoietin 1 have an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. The coiled coil domains mediate ligand homo-oligomerization and the fibrinogen-like domains mediate ligand activity. Recombinant human angiopoietin 2 has a molecular mass of approximately 66 kDa in SDS-PAGE under reducing and non-reducing conditions. Angiopoietin 2 probably acts as a natural antagonist for Ang1 and Tie2 both in vivo and in vitro. In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AGPT2; ANG-2; ANG2; angiopoietin-2a; angiopoietin-2B; Tie2-ligand
AGPT2; ANG2; ANGPT2