Invitrogen

Angiopoietin 2 Polyclonal Antibody

Product Image Gallery

Angiopoietin 2 Antibody — Figure EV1 Application of tissue microarrays ( TMA s) for the analysis of human glioma samples A, B Biopsies of 303 human gliomas ( WHO grade II , n = 16, WHO grade III , n = 35, and WHO grade IV , n = 252) were spotted on microscope slides (A) and processed for automated anti-Ang-2 immunohistochemistry (Ventana Benchmark platform) (B). Scale bar (B): 200 mum, inset: 50 mum. C Ang-2 expression was assessed by applying a semiquantitative scoring system (Harter et al , 2010 ). D Spatial expression of Ang-2 in brain specimens from glioblastoma patients was scored in normal-appearing gray matter ( NAGM ) ( n = 48), normal-appearing white matter ( NAWM ) ( n = 18), infiltration zone ( n = 39), and tumor center ( n = 62). Data information: In (C, D), for statistical analysis, Kruskal-Wallis test (followed by Dunn and apos;s post-test) was applied. *** P < 0.005. Whisker Box plots displaying median, 25-75 th percentile, upper and lower quartile.

Angiopoietin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Angiopoietin 2 in paraffin-embedded colon carcinoma using an Angiopoietin 2 polyclonal antibody (Product # PA5-27297) at a dilution of 1:250.

Angiopoietin 2 Antibody in Western Blot (WB) — Western blot analysis of Angiopoietin 2 in Mouse lung extracts (50 µg) and HUVEC cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with Angiopoietin 2 Polyclonal antibody (Product # PA5-27297) at a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Published Applications

Miscellaneous PubMed (MISC)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544773

Product Specific Information

PA5-27297 targets Angiopoietin 2 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.

The PA5-27297 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.