Invitrogen

Angiopoietin 2 Polyclonal Antibody

Angiopoietin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Angiopoietin 2 Antibody (PA5-27297) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Angiopoietin 2 (Product # PA5-27297) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Angiopoietin 2 Antibody in Western Blot (WB)

Angiopoietin 2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-27297

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Angiopoietin 2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544773

Product Specific Information

Recommended positive controls: HUVEC.

Predicted reactivity: Mouse (84%), Rat (86%), Dog (92%), Pig (92%), Chicken (85%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Direct Thrombin Inhibitor Dabigatran Compromises Pulmonary Endothelial Integrity in a Murine Model of Breast Cancer Metastasis to the Lungs; the Role of Platelets and Inflammation-Associated Haemostasis.

Frontiers in pharmacology

Smeda M,Stojak M,Przyborowski K,Sternak M,Suraj-Prazmowska J,Kus K,Derszniak K,Jasztal A,Kij A,Kurpinska A,Kieronska-Rudek A,Wojnar-Lason K,Buczek E,Mohaissen T,Chlopicki S

Published figure using Angiopoietin 2 polyclonal antibody (Product # PA5-27297) in Western Blot

Sat Mar 19 00:00:00 UTC 2022

Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged mice.

Frontiers in molecular biosciences

Smeda M,Jasztal A,Maleki EH,Bar A,Sternak M,Kwiatkowski G,Suraj-Prażmowska J,Proniewski B,Kieronska-Rudek A,Wojnar-Lason K,Skrzypek K,Majka M,Chrabaszcz K,Malek K,Chlopicki S

PA5-27297 was used in Western Blotting to indicate that age-dependent pre-existing endothelial dysfunction in the pulmonary endothelium of 40-week-old mice predisposed them to rapid EndMT progression in the presence of circulating 4T1 breast cancer cells what might contribute to a more severe metastatic breast cancer phenotype in these ageing mice compared to younger mice.

Fri Mar 03 00:00:00 UTC 2023

Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861.

Cancer research

Pedrosa AR,Bodrug N,Gomez-Escudero J,Carter EP,Reynolds LE,Georgiou PN,Fernandez I,Lees DM,Kostourou V,Alexopoulou AN,Batista S,Tavora B,Serrels B,Parsons M,Iskratsch T,Hodivala-Dilke KM

PA5-27297 was used in Western Blotting to establish the differential requirements of EC-FAK-Y397 and EC-FAK-Y861-phosphorylation in the regulation of EC-signalling and tumor angiogenesis in vivo.

Sun Sep 01 00:00:00 UTC 2019

Impact of Docetaxel on blood-brain barrier function and formation of breast cancer brain metastases.

Journal of experimental & clinical cancer research : CR

Bernatz S,Ilina EI,Devraj K,Harter PN,Mueller K,Kleber S,Braun Y,Penski C,Renner C,Halder R,Jennewein L,Solbach C,Thorsen F,Pestalozzi BC,Mischo A,Mittelbronn M

PA5-27297 was used in Immunohistochemistry to identify tubulin family members and tetraspanin-2 as potential contributors for the destabilization of the blood-brain barrier.

Tue Oct 29 00:00:00 UTC 2019

Impact of Docetaxel on blood-brain barrier function and formation of breast cancer brain metastases.

Journal of experimental & clinical cancer research : CR

Bernatz S,Ilina EI,Devraj K,Harter PN,Mueller K,Kleber S,Braun Y,Penski C,Renner C,Halder R,Jennewein L,Solbach C,Thorsen F,Pestalozzi BC,Mischo A,Mittelbronn M

PA5-27297 was used in Immunohistochemistry to identify tubulin family members and tetraspanin-2 as potential contributors for the destabilization of the blood-brain barrier.

Tue Oct 29 00:00:00 UTC 2019

Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour.

Oncotarget

Smeda M,Kieronska A,Proniewski B,Jasztal A,Selmi A,Wandzel K,Zakrzewska A,Wojcik T,Przyborowski K,Derszniak K,Stojak M,Kaczor D,Buczek E,Watala C,Wietrzyk J,Chlopicki S

PA5-27297 was used in Western Blotting to establish that distinct platelet-dependent mechanisms inhibited by ASA+Cl treatment promoted cancer malignancy and VM in the presence of primary tumour.

Tue Apr 03 00:00:00 UTC 2018

Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice.

Breast cancer research : BCR

Smeda M,Kieronska A,Adamski MG,Proniewski B,Sternak M,Mohaissen T,Przyborowski K,Derszniak K,Kaczor D,Stojak M,Buczek E,Jasztal A,Wietrzyk J,Chlopicki S

PA5-27297 was used in Western Blotting to suggest that early endothelial dysfunction featured by NO deficiency rather than EndMT, might represent a primary regulatory target to prevent early pulmonary metastasis.

Fri Aug 03 00:00:00 UTC 2018

Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma.

EMBO molecular medicine

Scholz A,Harter PN,Cremer S,Yalcin BH,Gurnik S,Yamaji M,Di Tacchio M,Sommer K,Baumgarten P,Bähr O,Steinbach JP,Trojan J,Glas M,Herrlinger U,Krex D,Meinhardt M,Weyerbrock A,Timmer M,Goldbrunner R,Deckert M,Braun C,Schittenhelm J,Frueh JT,Ullrich E,Mittelbronn M,Plate KH,Reiss Y

Published figure using Angiopoietin 2 polyclonal antibody (Product # PA5-27297) in Immunofluorescence

Fri Jan 01 00:00:00 UTC 2016

Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma.

EMBO molecular medicine

Published figure using Angiopoietin 2 polyclonal antibody (Product # PA5-27297) in Immunofluorescence

Fri Jan 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: ANG-2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; Tie2-ligand

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Gene Aliases: AGPT2; Ang-2; ANG2; ANGPT2

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UniProt ID: (Human) O15123, (Mouse) O35608, (Rat) O35462

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Entrez Gene ID: (Human) 285, (Mouse) 11601, (Rat) 89805

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Function(s)

receptor binding protein binding receptor tyrosine kinase binding metal ion binding vascular endothelial growth factor receptor binding molecular_function intercellular signal molecule

Process(es)

angiogenesis response to hypoxia signal transduction germ cell development response to radiation response to mechanical stimulus response to glucose negative regulation of cell-substrate adhesion response to organic cyclic compound response to activity negative regulation of angiogenesis organ regeneration negative regulation of blood vessel endothelial cell migration positive regulation of angiogenesis Tie signaling pathway leukocyte migration negative regulation of positive chemotaxis maternal process involved in female pregnancy cellular response to growth factor stimulus glomerulus vasculature development blood vessel remodeling transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development endoderm development hemopoiesis cell differentiation regulation of angiogenesis blood vessel morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Angiopoietin 2 Polyclonal Antibody

Angiopoietin 2 Antibody (PA5-27297) in IHC (P)

Product Details

PA5-27297

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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