Invitrogen

Angiopoietin 2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4 Published Figures

5 References

View all (19) Angiopoietin 2 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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Angiopoietin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Angiopoietin 2 (Product # PA5-27297) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Angiopoietin 2 Antibody in Western Blot (WB) — Western blot was performed using Anti-Angiopoietin 2 Polyclonal Antibody (Product # PA5-27297) and a 65 kDa band corresponding to Angiopoietin-2 was observed across the cells and tissues tested. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), HAEC (Lane 2), HeLa (Lane 3), Mouse Aorta (Lane 4), Rat Aorta (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Angiopoietin 2 Antibody in Immunohistochemistry (IHC) — Figure EV1 Application of tissue microarrays ( TMA s) for the analysis of human glioma samples A, B Biopsies of 303 human gliomas ( WHO grade II , n = 16, WHO grade III , n = 35, and WHO grade IV , n = 252) were spotted on microscope slides (A) and processed for automated anti-Ang-2 immunohistochemistry (Ventana Benchmark platform) (B). Scale bar (B): 200 mum, inset: 50 mum. C Ang-2 expression was assessed by applying a semiquantitative scoring system (Harter et al , 2010 ). D Spatial expression of Ang-2 in brain specimens from glioblastoma patients was scored in normal-appearing gray matter ( NAGM ) ( n = 48), normal-appearing white matter ( NAWM ) ( n = 18), infiltration zone ( n = 39), and tumor center ( n = 62). Data information: In (C, D), for statistical analysis, Kruskal-Wallis test (followed by Dunn's post-test) was applied. *** P < 0.005. Whisker Box plots displaying median, 25-75 th percentile, upper and lower quartile.

Angiopoietin 2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with Angiopoietin-2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Angiopoietin 2 Polyclonal Antibody (Product # PA5-27297). {KD}

Product Details

PA5-27297

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.64 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544773

Product Specific Information

Recommended positive controls: HUVEC.

Predicted reactivity: Mouse (84%), Rat (86%), Dog (92%), Pig (92%), Chicken (85%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Direct Thrombin Inhibitor Dabigatran Compromises Pulmonary Endothelial Integrity in a Murine Model of Breast Cancer Metastasis to the Lungs; the Role of Platelets and Inflammation-Associated Haemostasis.

Frontiers in pharmacology

Smeda M,Stojak M,Przyborowski K,Sternak M,Suraj-Prazmowska J,Kus K,Derszniak K,Jasztal A,Kij A,Kurpinska A,Kieronska-Rudek A,Wojnar-Lason K,Buczek E,Mohaissen T,Chlopicki S

Published figure using Angiopoietin 2 polyclonal antibody (Product # PA5-27297) in Western Blot

Sat Mar 19 00:00:00 EDT 2022

Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861.

Cancer research

Pedrosa AR,Bodrug N,Gomez-Escudero J,Carter EP,Reynolds LE,Georgiou PN,Fernandez I,Lees DM,Kostourou V,Alexopoulou AN,Batista S,Tavora B,Serrels B,Parsons M,Iskratsch T,Hodivala-Dilke KM

PA5-27297 was used in Western Blotting to establish the differential requirements of EC-FAK-Y397 and EC-FAK-Y861-phosphorylation in the regulation of EC-signalling and tumor angiogenesis in vivo.

Sun Sep 01 00:00:00 EDT 2019

Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour.

Oncotarget

Smeda M,Kieronska A,Proniewski B,Jasztal A,Selmi A,Wandzel K,Zakrzewska A,Wojcik T,Przyborowski K,Derszniak K,Stojak M,Kaczor D,Buczek E,Watala C,Wietrzyk J,Chlopicki S

PA5-27297 was used in Western Blotting to establish that distinct platelet-dependent mechanisms inhibited by ASA+Cl treatment promoted cancer malignancy and VM in the presence of primary tumour.

Tue Apr 03 00:00:00 EDT 2018

Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma.

EMBO molecular medicine

Scholz A,Harter PN,Cremer S,Yalcin BH,Gurnik S,Yamaji M,Di Tacchio M,Sommer K,Baumgarten P,Bähr O,Steinbach JP,Trojan J,Glas M,Herrlinger U,Krex D,Meinhardt M,Weyerbrock A,Timmer M,Goldbrunner R,Deckert M,Braun C,Schittenhelm J,Frueh JT,Ullrich E,Mittelbronn M,Plate KH,Reiss Y