Invitrogen

Angiopoietin 2 Polyclonal Antibody

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Angiopoietin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Angiopoietin 2 in paraffin-embedded colon carcinoma using an Angiopoietin 2 polyclonal antibody (Product # PA5-27297) at a dilution of 1:250.

Angiopoietin 2 Antibody in Western Blot (WB) — Western Blot analysis of Angiopoietin 2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Angiopoietin 2 Polyclonal Antibody (Product # PA5-27297) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Angiopoietin 2 Antibody — Figure EV1 Application of tissue microarrays ( TMA s) for the analysis of human glioma samples A, B Biopsies of 303 human gliomas ( WHO grade II , n = 16, WHO grade III , n = 35, and WHO grade IV , n = 252) were spotted on microscope slides (A) and processed for automated anti-Ang-2 immunohistochemistry (Ventana Benchmark platform) (B). Scale bar (B): 200 mum, inset: 50 mum. C Ang-2 expression was assessed by applying a semiquantitative scoring system (Harter et al , 2010 ). D Spatial expression of Ang-2 in brain specimens from glioblastoma patients was scored in normal-appearing gray matter ( NAGM ) ( n = 48), normal-appearing white matter ( NAWM ) ( n = 18), infiltration zone ( n = 39), and tumor center ( n = 62). Data information: In (C, D), for statistical analysis, Kruskal-Wallis test (followed by Dunn and apos;s post-test) was applied. *** P < 0.005. Whisker Box plots displaying median, 25-75 th percentile, upper and lower quartile.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Immunofluorescence (IF)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.64 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544773

Product Specific Information

Recommended positive controls: HUVEC.

Predicted reactivity: Mouse (84%), Rat (86%), Dog (92%), Pig (92%), Chicken (85%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Angiopoietin-2 (ANGPT2) is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.