|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-27297 targets Angiopoietin 2 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-27297 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 496 of Human Angiopoietin 2.
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Endothelial cell-derived angiopoietin-2 is a therapeutic target in treatment-naive and bevacizumab-resistant glioblastoma.
PA5-27297 was used in immunohistochemistry to report angiopoietin-2 as a target in both naive and bevacizumab-treated glioblastoma
|Scholz A,Harter PN,Cremer S,Yalcin BH,Gurnik S,Yamaji M,Di Tacchio M,Sommer K,Baumgarten P,Bähr O,Steinbach JP,Trojan J,Glas M,Herrlinger U,Krex D,Meinhardt M,Weyerbrock A,Timmer M,Goldbrunner R,Deckert M,Braun C,Schittenhelm J,Frueh JT,Ullrich E,Mittelbronn M,Plate KH,Reiss Y||EMBO molecular medicine (8:39)||2016|