Invitrogen

Annexin A3 Monoclonal Antibody (A1F7)

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Annexin A3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Annexin A3 in HT-29 cells using a Annexin A3 monoclonal antibody (Product #MA5-36106). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

Annexin A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Annexin A3 in paraffin-embedded human pancreas tissue using a monoclonal antibody (Product #MA5-36106). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Annexin A3 Antibody in Western Blot (WB) — Western blot analysis of Annexin A3 using a Annexin A3 monoclonal antibody (Product #MA5-36106). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Annexin A3 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Annexin A3 using A431 cells and a Annexin A3 monoclonal antibody (Product #MA5-36106). The cells were fixed, permeabilized and stained with the primary antibody at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1:1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-36106

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:100	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	A1F7
Immunogen	Recombinant protein within Human Annexin A3 aa 1-323 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 35-alpha calcimedin; Annexin 3; Annexin A3; Annexin III; Annexin III (lipocortin III); annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha); Annexin-3; Annexin3; ANXA 3; calcimedin 35-alpha; Inositol 1,2-cyclic phosphate 2-phosphohydrolase; Lipocortin III; PAP-III; Placental anticoagulant protein III

Gene Aliases: ANX3; ANXA3

UniProt ID: (Human) P12429, (Mouse) O35639

Entrez Gene ID: (Human) 306, (Mouse) 11745

Molecular Function: calcium-binding protein