Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence analysis of Annexin-1 Antibody was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Annexin-1 Antibody (713400) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length human recombinant Annexin I protein purified from yeast.|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Systemic gene dysregulation in classical Galactosaemia: Is there a central mechanism?
71-3400 was used in immunocytochemistry to identify dysregulated genes in classical galactosaemia.
|Coss KP,Treacy EP,Cotter EJ,Knerr I,Murray DW,Shin YS,Doran PP||Molecular genetics and metabolism (113:177)||2014|
MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation.
71-3400 was used in western blot to discuss the clinical applications of using microRNA biomarkers in head and neck squamous cell carcinomas.
|Severino P,Brüggemann H,Andreghetto FM,Camps C,Klingbeil Mde F,de Pereira WO,Soares RM,Moyses R,Wünsch-Filho V,Mathor MB,Nunes FD,Ragoussis J,Tajara EH||BMC cancer (13:null)||2014|
PAX2 Expression in Ovarian Cancer.
71-3400 was used in western blot to determine the functional role of PAX2 in ovarian cancer.
|Song H,Kwan SY,Izaguirre DI,Zu Z,Tsang YT,Tung CS,King ER,Mok SC,Gershenson DM,Wong KK||International journal of molecular sciences (14:6090)||2013|
|Mouse||Not Cited||Regulation of cytosolic phospholipase A2 phosphorylation by proteolytic cleavage of annexin A1 in activated mast cells.||Kwon JH,Lee JH,Kim KS,Chung YW,Kim IY||Journal of immunology (Baltimore, Md. : 1950) (188:5665)||2012|
|Rat||Not Cited||Anti-inflammatory mechanisms of the annexin A1 protein and its mimetic peptide Ac2-26 in models of ocular inflammation in vivo and in vitro.||Girol AP,Mimura KK,Drewes CC,Bolonheis SM,Solito E,Farsky SH,Gil CD,Oliani SM||Journal of immunology (Baltimore, Md. : 1950) (190:5689)||2013|
||Regulation of cytosolic phospholipase A2 phosphorylation by proteolytic cleavage of annexin A1 in activated mast cells.||Kwon JH,Lee JH,Kim KS,Chung YW,Kim IY||Journal of immunology (Baltimore, Md. : 1950) (188:5665)||2012|
Annexin 1 (p35) (Lipocortin 1); annexin A1; annexin I; annexin I (lipocortin I); annexin-1; ANXA1; calpactin II; calpactin-2; chromobindin-9; lipocortin 1; lipocortin I; p35; phospholipase A2 inhibitory protein
Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35