Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Breast Carcinoma tissue using PA5-19711, anti-Annexin II antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Annexin II.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with rat, sheep, cow, dog and pig based on sequence homology.
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
33-kDa calcimedin; 33-kDa lymphocyte Ca<2+>- binding protein; 33kDa lymphocyte Ca<2+>- binding protein; 36-kDa calelectrin; annexin A2; annexin II; annexin-2; ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, annexin II; calpactin I heavy chain; calpactin I heavy polypeptide; calpactin-1 heavy chain; chromobindin 8; chromobindin-8; epididymis secretory protein Li 270; lipocortin II; p36; PAP-IV; placental anticoagulant protein IV; protein I
ANX2; ANX2L4; ANXA2; AW215814; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV