Western blot analysis of NIH 3T3 whole cell lysate using PA5-19424, Apc7 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19424 staining of MEF1 whole cell lysate at a dilution of 1 ug/ml (Lane 2). PA5-19424 staining of mouse brain tissue lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apc7|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 39 kDa and 45 kDa.
This gene encodes a tetratricopeptide repeat containing component of the anaphase promoting complex/cyclosome , a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with certain transcription coactivators. Multiple transcript variants encoding different isoforms have been found for this gene.
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