Invitrogen

ApoA1 Polyclonal Antibody

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ApoA1 Antibody in Western Blot (WB) — Western blot was performed using Anti-ApoA1 Polyclonal Antibody(Product # PA5-19784) and a 25kDa band corresponding to ApoA1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with FLI, 10 uM, 4 Hrs. (Lane 2), Hep G2 treated with PTI, 1X, 4Hrs. (Lane 3), SH-SY5Y (Lane 4), SH-SY5Y treated with FLI, 10 uM, 4 Hrs (Lane 5), SH-SY5Y treated with PTI, 1X, 4Hrs. (Lane 6), Empty lane (Lane 7) and Human Plasma (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Expression of APOA1 was found to be higher in Hep G2 cell line as compared to SH-SY 5Y.

ApoA1 Antibody in Western Blot (WB) — Western blot analysis of Human Lymph node Tissue Lysate using Product # PA5-19784, Apolipoprotein A I primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Human Testis Tissue Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Product Details

PA5-19784

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1-5 µg/mL	-
ELISA (ELISA)	0.5 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Apolipoprotein A I. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10989874

Product Specific Information

This antibody is predicted to react with dog, pig and baboon based on sequence homology.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.

The Journal of clinical investigation

Conlon DM,Thomas T,Fedotova T,Hernandez-Ono A,Di Paolo G,Chan RB,Ruggles K,Gibeley S,Liu J,Ginsberg HN

PA519784 was used in western blot to study the effects of apolipoprotein B inhibition on VLDL levels and hepatic lipids in mice.

Mon Oct 03 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7

Gene Aliases: apo(a); APOA1

UniProt ID: (Human) P02647

Entrez Gene ID: (Human) 335

Molecular Function: apolipoprotein

retinoid metabolic process regulation of protein phosphorylation endothelial cell proliferation platelet degranulation negative regulation of cytokine secretion involved in immune response phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process receptor-mediated endocytosis transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway integrin-mediated signaling pathway response to nutrient cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling peripheral nervous system axon regeneration protein oxidation peptidyl-methionine modification triglyceride catabolic process lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development organ regeneration neuron projection regeneration regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process response to drug cholesterol homeostasis blood vessel endothelial cell migration response to estrogen reverse cholesterol transport cellular protein metabolic process positive regulation of fatty acid biosynthetic process negative regulation of interleukin-1 beta secretion negative regulation of inflammatory response protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport positive regulation of hydrolase activity positive regulation of stress fiber assembly transmembrane transport phospholipid homeostasis negative regulation of lipase activity negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis ERK1 and ERK2 cascade cholesterol import positive regulation of substrate adhesion-dependent cell spreading

Process(es)

beta-amyloid binding protein binding phospholipid binding phospholipid transporter activity high-density lipoprotein particle binding cholesterol binding cholesterol transporter activity enzyme binding phosphatidylcholine binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding