Invitrogen

ApoA1 Polyclonal Antibody

2 Published Figures

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ApoA1 Antibody in Western Blot (WB) — Western Blot analysis of ApoA1 in chicken liver tissue lysate with ApoA1 Polyclonal Antibody (Product # PA5-21166) at 1 µg/mL .

ApoA1 Antibody in Western Blot (WB) — Figure 6 The purity of EV preparation. (A) Western blot comparing protein levels in three biological replicates of ABEVs (EV) and crude brainstem lysates (BS). Neural cell adhesion molecule (NCAM), a positive marker for EVs released by neural tissue, was enriched in ABEVs. In contrast, negative markers for EVs, namely calnexin (CANX), APOA1, and VDAC, showed either no difference in protein amount between brainstem lysates and ABEVs (CANX and APOA1) or showed a decreased expression in ABEVs (VDAC). Both procaspase-3 (PC3) and cleaved caspase-3 (CC3) were negatively enriched in ABEVs compared to brainstem lysates. (B) All proteins were normalized to total protein stain as a loading control. (C) Quantification of normalized protein levels from A. All p -values are derived from two-tailed Student's t -tests except for procaspase-3, for which Welch's heteroscedastic t -test was necessary. (D) Proportionally accurate Venn diagram of proteins identified in each ABEV replicate. The substantial triple-overlap reflects a high degree of conservation among the proteomes of the individual ABEV replicates. (E) Comparison of ExoCarta dataset counts of brainstem proteins and ABEV proteins found in one, two, or three replicates. The number of EV proteomic datasets containing each protein on the EV database ExoCarta (""ExoCarta dataset counts"") was used to estimate the frequency that proteins are found in EVs. ABEV proteins had higher ExoCarta dataset counts than brainstem proteins regardless

ApoA1 Antibody in Western Blot (WB) — Published figure using ApoA1 polyclonal antibody (Product # PA5-21166) in Western Blot

Product Details

PA5-21166

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Human

Published species

Chicken, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 19 amino acid peptide near the carboxy terminus of human ApoA1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_11154087

Product Specific Information

A suggested positive control is chicken liver lysate.

PA5-21166 can be used with blocking peptide PEP-1280.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development.

Frontiers in cellular neuroscience

Weghorst F,Mirzakhanyan Y,Samimi K,Dhillon M,Barzik M,Cunningham LL,Gershon PD,Cramer KS

PA5-21166 was used in Western Blot to identify proteolytic substrates of caspase-3 during chick auditory brainstem development.

Fri Aug 26 00:00:00 EDT 2022

Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development.

Frontiers in cellular neuroscience

Weghorst F,Mirzakhanyan Y,Samimi K,Dhillon M,Barzik M,Cunningham LL,Gershon PD,Cramer KS

PA5-21166 was used in Western Blot to identify proteolytic substrates of caspase-3 during chick auditory brainstem development.

Fri Aug 26 00:00:00 EDT 2022

Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer.

The Journal of biological chemistry

Justesen BH,Hansen RW,Martens HJ,Theorin L,Palmgren MG,Martinez KL,Pomorski TG,Fuglsang AT

Published figure using ApoA1 polyclonal antibody (Product # PA5-21166) in Western Blot

Fri Sep 13 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; apoA-I; ApoAI; Apolipoprotein; Apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; prepro-apolipoprotein-AI; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7

Gene Aliases: apo(a); APOA1

UniProt ID: (Chicken) P08250, (Human) P02647

Entrez Gene ID: (Chicken) 396536, (Human) 335

Molecular Function: apolipoprotein

regulation of protein phosphorylation endothelial cell proliferation phosphatidylcholine biosynthetic process cholesterol biosynthetic process G-protein coupled receptor signaling pathway integrin-mediated signaling pathway glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of cholesterol efflux positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling protein oxidation peptidyl-methionine modification lipid storage regulation of intestinal cholesterol absorption adrenal gland development regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion very-low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport myoblast differentiation positive regulation of fatty acid biosynthetic process phosphatidylcholine metabolic process positive regulation of lipid biosynthetic process negative regulation of inflammatory response positive regulation of phagocytosis protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport negative regulation of hydrolase activity positive regulation of stress fiber assembly regulation of cell cycle phospholipid homeostasis negative regulation of cell adhesion molecule production triglyceride homeostasis cholesterol import intermembrane lipid transfer positive regulation of substrate adhesion-dependent cell spreading negative regulation of lung ciliated cell differentiation positive regulation of phospholipid efflux retinoid metabolic process platelet degranulation negative regulation of cytokine secretion involved in immune response phospholipid metabolic process receptor-mediated endocytosis transforming growth factor beta receptor signaling pathway response to nutrient cholesterol metabolic process peripheral nervous system axon regeneration triglyceride catabolic process cholesterol transport organ regeneration neuron projection regeneration high-density lipoprotein particle clearance response to drug response to estrogen cellular protein metabolic process negative regulation of interleukin-1 beta secretion positive regulation of hydrolase activity transmembrane transport negative regulation of lipase activity negative regulation of response to cytokine stimulus ERK1 and ERK2 cascade

Process(es)

beta-amyloid binding protein binding phospholipid binding high-density lipoprotein particle binding cholesterol binding enzyme binding heat shock protein binding phosphatidylcholine binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding intermembrane cholesterol transfer activity phospholipid transporter activity cholesterol transporter activity apolipoprotein receptor binding lipase inhibitor activity