|Tested species reactivity||Chicken, Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide near the carboxy terminus of human ApoA1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control is chicken liver lysate.
PA5-21166 can be used with blocking peptide PEP-1280.
Apolipoprotein A1 (ApoA1) is the major protein component of high density lipoprotein (HDL) in plasma, which is correlated with cardiovascular disease. ApoA1 is synthesized in the liver and small intestine and promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT), the enzyme responsible for the formation of most plasma cholesteryl esters. Defects in ApoA1 are associated with HDL deficiency, Tangier disease, and systemic non-neuropathic amyloidosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer.
PA5-21166 was used in western blot to study the monomeric nature of the Arabidopsis thaliana P-type H(+)-ATPase isoform 2 functionally reconstituted into nanodiscs
|Justesen BH,Hansen RW,Martens HJ,Theorin L,Palmgren MG,Martinez KL,Pomorski TG,Fuglsang AT||The Journal of biological chemistry (288:26419)||2013|