Invitrogen

ApoA1 Polyclonal Antibody

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ApoA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Apolipoprotein A1 in paraffin-embedded Huh7 xenograft using an Apolipoprotein A1 polyclonal antibody (Product # PA5-29557) at a dilution of 1:500.

ApoA1 Antibody in Western Blot (WB) — Western blot analysis of Apolipoprotein A1 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Apolipoprotein A1 polyclonal antibody (Product # PA5-29557) at a dilution of 1:2000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 267 of Human Apolipoprotein A1

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547033

Product Specific Information

PA5-29557 targets Apolipoprotein A1 in IHC (P) and WB applications and shows reactivity with Human samples.

The PA5-29557 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 267 of Human Apolipoprotein A1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apo-AI; apoA-I; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

Gene Aliases: apo(a); APOA1

UniProt ID: (Human) P02647

Entrez Gene ID: (Human) 335

retinoid metabolic process regulation of protein phosphorylation endothelial cell proliferation platelet degranulation negative regulation of cytokine secretion involved in immune response phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process receptor-mediated endocytosis transforming growth factor beta receptor signaling pathway G-protein coupled receptor signaling pathway integrin-mediated signaling pathway response to nutrient cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling peripheral nervous system axon regeneration protein oxidation peptidyl-methionine modification triglyceride catabolic process lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development organ regeneration neuron projection regeneration regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion high-density lipoprotein particle remodeling high-density lipoprotein particle assembly high-density lipoprotein particle clearance positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process response to drug cholesterol homeostasis blood vessel endothelial cell migration response to estrogen reverse cholesterol transport cellular protein metabolic process positive regulation of fatty acid biosynthetic process negative regulation of interleukin-1 beta secretion negative regulation of inflammatory response protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport positive regulation of hydrolase activity positive regulation of stress fiber assembly transmembrane transport phospholipid homeostasis negative regulation of lipase activity negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis ERK1 and ERK2 cascade cholesterol import positive regulation of substrate adhesion-dependent cell spreading

Process(es)

beta-amyloid binding protein binding phospholipid binding phospholipid transporter activity high-density lipoprotein particle binding cholesterol binding cholesterol transporter activity enzyme binding phosphatidylcholine binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding