Invitrogen

ApoA1 Polyclonal Antibody

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Questions & Answers

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ApoA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ApoA1 on paraffin-embedded mouse kidney tissue. Sample was incubated with ApoA1 polyclonal antibody (Product# PA5-78798) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

ApoA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ApoA1 on paraffin-embedded mouse liver tissue. Sample was incubated with ApoA1 polyclonal antibody (Product# PA5-78798) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

ApoA1 Antibody in Western Blot (WB) — Western blot was performed using Anti-ApoA1 Polyclonal Antibody (Product # PA5-78798) and a 25kDa band corresponding to ApoA1 was observed across tissues tested. Whole cell extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Brain (Lane 2), Mouse Lung (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL concentration) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).Expression of APOA1 was found to be higher in Mouse liver as compared to Mouse brain and Mouse lung.

ApoA1 Antibody in Western Blot (WB) — Western blot analysis of ApoA1 in mouse liver extract (lane 1). Sample was incubated with ApoA1 polyclonal antibody (Product # PA5-78798) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived mouse APOA1 recombinant protein (Position: D25-Q264).

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

RRID

AB_2745914

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7

Gene Aliases: Alp-1; apo-AI; Apoa-1; apoA-I; Apoa1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2

UniProt ID: (Mouse) Q00623

Entrez Gene ID: (Mouse) 11806

Molecular Function: apolipoprotein

regulation of protein phosphorylation endothelial cell proliferation negative regulation of cytokine secretion involved in immune response lipid metabolic process phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process transport lipid transport G-protein coupled receptor signaling pathway integrin-mediated signaling pathway protein localization steroid metabolic process cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling phospholipid transport protein oxidation peptidyl-methionine modification triglyceride catabolic process lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development organ regeneration axon regeneration regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion very-low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport positive regulation of fatty acid biosynthetic process phosphatidylcholine metabolic process negative regulation of interleukin-1 beta secretion negative regulation of inflammatory response protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport positive regulation of hydrolase activity negative regulation of hydrolase activity positive regulation of transferase activity positive regulation of stress fiber assembly phospholipid homeostasis negative regulation of lipase activity negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis cholesterol import positive regulation of substrate adhesion-dependent cell spreading

Process(es)

beta-amyloid binding lipid transporter activity protein binding phospholipid binding phospholipid transporter activity high-density lipoprotein particle binding lipid binding cholesterol binding cholesterol transporter activity enzyme binding phosphatidylcholine binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding lipoprotein particle binding