By sandwich ELISA, MA5-14670 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MA5-14670 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14670, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MA5-14670 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14670 is also recommended for detecting endogenous Apo A-1 by Western blot.
Product MA514670 is a smaller package size of MIA1404 (formerly sold as a Seradyn product).
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1-Sep; 2-Sep; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
Gene Aliases: apo(a); APOA1
UniProt ID: (Human) P02647
Entrez Gene ID: (Human) 335
Molecular Function: apolipoprotein