Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
2 µg/mL | - |
ELISA (ELISA) |
1 µg/mL | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
Radioimmune Assays (RIA) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
513 |
Immunogen |
Purified human plasma Apo A-1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
5 mg/mL |
Purification |
Ion-exchange chromatography |
Storage buffer |
PBS, pH 7.4 |
Contains |
0.09% sodium azide |
Storage conditions |
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
RRID |
AB_11151929 |
By sandwich ELISA, MIA1404 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1404 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1404, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MIA1404 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1404 is also recommended for detecting endogenous Apo A-1 by Western blot.
MIA1404 was formerly sold as a Seradyn product.
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
Gene Aliases: Alp-1; apo(a); apo-AI; Apoa-1; apoA-I; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2
UniProt ID: (Human) P02647, (Mouse) Q00623
Entrez Gene ID: (Human) 335, (Mouse) 11806
Molecular Function:
apolipoprotein
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