This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
By sandwich ELISA, MIA1401 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MIA1401 is used for detection.MIA1401 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1401, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MIA1401 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1401 may not be as successful for detecting endogenous Apo A-1 by Western blot.
MIA1401 was formerly sold as a Seradyn product.
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1-Sep; 2-Sep; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; apolipoprotein A-1; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
Gene Aliases: Alp-1; apo(a); apo-AI; Apoa-1; apoA-I; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2