|Tested species reactivity||Human|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Native Apo-A1 from human plasma.|
|Storage buffer||PBS/BBS with 1% dextran, 1% mannitol|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:40|
|Western Blot (WB)||1/2000 - 1/8,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-83002 reacts with both free human Apo-A1 and High Density Lipoprotein (HDL) bearing Apo-A1, but does not cross-react with ApoE, ApoB or Albumin.
Reconstitute with 1 ml of distilled water. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
Apolipoprotein A-1, also known as Apo-A1, is a lipid-binding protein which enables the transport of dietary lipids for storage, metabolism and secretion. Apo-A1 plays an important part in the removal of cholesterol from cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration.
MA1-83002 was used in immunoprecipitation to investigate the important roles of Trp residues in the function of GCAP1 and its conformational changes mediated by calcium binding
|Sokal I,Otto-Bruc AE,Surgucheva I,Verlinde CL,Wang CK,Baehr W,Palczewski K||The Journal of biological chemistry (274:19829)||1999|
Functional reconstitution of photoreceptor guanylate cyclase with native and mutant forms of guanylate cyclase-activating protein 1.
MA1-83002 was used in immunoprecipitation to investigate the mechanism for the restoration of the functions of retGC1 by GCAP1
|Otto-Bruc A,Buczylko J,Surgucheva I,Subbaraya I,Rudnicka-Nawrot M,Crabb JW,Arendt A,Hargrave PA,Baehr W,Palczewski K||Biochemistry (36:4295)||1997|
Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxin.
MA1-83002 was used in western blot to investigate the influence of calcium on rhodopsin phosphorylation in retina
|Otto-Bruc AE,Fariss RN,Van Hooser JP,Palczewski K||Proceedings of the National Academy of Sciences of the United States of America (95:15014)||1998|
Localization of guanylate cyclase-activating protein 2 in mammalian retinas.
MA1-83002 was used in western blot to investigate the distribution of guanylate cyclase-activating protein 2 in human, monkey and bovine retinas
|Otto-Bruc A,Fariss RN,Haeseleer F,Huang J,Buczy¿ko J,Surgucheva I,Baehr W,Milam AH,Palczewski K||Proceedings of the National Academy of Sciences of the United States of America (94:4727)||1997|
Molecular cloning and characterization of retinal photoreceptor guanylyl cyclase-activating protein.
MA1-83002 was used in blocking/activating experiment to clone guanylyl cyclase-activating protein and to investigate its functional properties
|Palczewski K,Subbaraya I,Gorczyca WA,Helekar BS,Ruiz CC,Ohguro H,Huang J,Zhao X,Crabb JW,Johnson RS||Neuron (13:395)||1994|