|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Native human apoLipoprotein type A-II.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26900 detects Apolipoprotein A II from human samples.
PA1-26900 has been successfully used in immunohistochemistry (paraffin), ELISA, immunoprecipitation, and Western blot procedures.
The PA1-26900 immunogen is purified, full length, native apoLipoprotein type A-II isolated from human plasma by density gradient centrifugation followed by HPLC purification.
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL, and VLDL particles of human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins ( especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease that measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteis are derived from human plasma by density gradient ultracentrifugation and HPLC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.