|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified, full length, native apoLipoprotein type B.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26901 detects Apolipoprotein B from human samples.
PA1-26901 has been successfully used in immunohistochemistry, ELISA, immunoprecipitation, and Western blot procedures.
The PA1-26901 immunogen is purified, full length, native apoLipoprotein type B.
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.