|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified full length native apolipoprotein type C-III.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8, with 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-22562 detects apolipoprotein C3 in human samples.
PA1-22562 has been successfully used in ELISA, immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. Typically there is less than 1% cross-reactivity against other apolipoprotein determined in ELISA
The PA1-22562 immunogen is purified full length native apolipoprotein type C-III.
Store vial at 4°C for short-term use. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing.
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.