|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified, full length, native apoLipoprotein type J.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 7.2, with 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-26903 detects Apolipoprotein J from human samples.
PA1-26903 has been successfully used in immunoprecipitation, immunohistochemistry, ELISA, and Western blot procedures.
The PA1-26903 immunogen is purified, full length, native apoLipoprotein type J.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
At least 9 distinct polymorphic form of apolopoproteins are know. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL, and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease then measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proteomic analysis of electronegative low-density lipoprotein.
PA1-26903 was used in western blot to profile the protein composition of electronegative low-density lipoprotein
|Bancells C,Canals F,Benítez S,Colomé N,Julve J,Ordóñez-Llanos J,Sánchez-Quesada JL||Journal of lipid research (51:3508)||2010|