Invitrogen

Apolipoprotein J Polyclonal Antibody

Product Image Gallery

Apolipoprotein J Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Apolipoprotein J in HeLa cells using a Apolipoprotein J monoclonal antibody (Product # PA5-19020) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Apolipoprotein J Antibody in Western Blot (WB) — Western blot analysis of Apolipoprotein J by a Apolipoprotein J monoclonal antibody (Product # PA5-19020) at a concentration of 0.3 µg/mL. Human Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

Assay Dependent

Western Blot (WB)

0.1-1 µg/mL

Product Specifications

Species Reactivity

Human, Rat

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (EKALQEYRRKSRAE) corresponding to the C-terminus amino acids of Clu

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10986117

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: Aging-associated gene 4 protein; aging-associated protein 4; AI893575; Apo-J; ApoJ; ApoJalpha; ApoJbeta; Apolipoprotein J; CLI; Clu; Clusterin; Clusterin alpha chain; Clusterin beta chain; Complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; Complement-associated protein SP-40,40; D14Ucla3; DAG; Dimeric acid glycoprotein; Ku70-binding protein 1; NA1/NA2; SGP-2; Sgp2; SP-40; Sugp-2; Sulfated glycoprotein 2; testosterone repressed prostate message; Testosterone repressed prostate message 2; testosterone-repressed prostate message; Testosterone-repressed prostate message 2; testostrone-repressed prostate message 2; TRPM-2

Gene Aliases: AAG4; APO-J; APOJ; CLI; CLU; CLU1; CLU2; DAG; KUB1; NA1/NA2; RATTRPM2B; SGP-2; SGP2; SP-40; SP40; TRPM-2; TRPM2; TRPM2B; Trpmb

UniProt ID: (Human) P10909, (Rat) P05371

Entrez Gene ID: (Human) 1191, (Rat) 24854

Function(s)