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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 220-449 of human CLU |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
3.4 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2805886 |
Immunogen sequence: FPKSRIVRSL MPFSPYEPLN FHAMFQPFLE MIHEAQQAMD IHFHSPAFQH PPTEFIREGD DDRTVCREIR HNSTGCLRMK DQCDKCREIL SVDCSTNNPS QAKLRRELDE SLQVAERLTR KYNELLKSYQ WKMLNTSSLL EQLNEQFNWV SRLANLTQGE DQYYLRVTTV ASHTSDSDVP SGVTEVVVKL FDSDPITVTV PVEVSRKNPK FMETVAEKAL QEYRKKHREE; Positive Samples: A-549, HepG2, SW480, HeLa, 22Rv1, Mouse liver; Cellular Location: Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Endoplasmic reticulum, Microsome, Mitochondrion membrane, Nucleus, Peripheral membrane protein, chromaffin granule, cytosol, secretory vesicle
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Aging associated protein 4; Aging-associated gene 4 protein; aging-associated protein 4; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J α; Apo J β; Apo Ja; Apo Jb; Apo-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ α; ApoJ β; ApoJa; ApoJalpha; ApoJb; ApoJbeta; Apolipoprotein J; CLI; Clusterin; Clusterin alpha / beta chain; Clusterin alpha chain; Clusterin beta chain; Clustrin; Complement associated protein SP40; Complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; Complement-associated protein SP-40,40; DAG; Dimeric acid glycoprotein; GP80; HGNC:2095; Ku70-binding protein 1; Ku70-binding protein 1 (KUB1); MGC24903; NA1/NA2; SGP-2; Sulfated glycoprotein 2; Sulfated glycoprotein 2 (SGP2); testosterone repressed prostate message; Testosterone repressed prostate message 2; Testosterone repressed prostate message 2 (TPRM2B); testosterone repressed prostate message-2; testosterone-repressed prostate message; Testosterone-repressed prostate message 2; testostrone-repressed prostate message 2; TRPM-2
Gene Aliases: AAG4; AI893575; APO-J; APOJ; CLI; CLU; CLU1; CLU2; D14Ucla3; DAG; KUB1; Msgp-2; NA1/NA2; RATTRPM2B; SGP-2; SGP2; SP-40; SP40; Sugp-2; TRPM-2; TRPM2; TRPM2B; Trpmb
UniProt ID: (Human) P10909, (Rat) P05371, (Mouse) Q06890
Entrez Gene ID: (Human) 1191, (Rat) 24854, (Mouse) 12759
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