Immunofluorescent analysis of Apoptosis-enhancing nuclease showing staining in the nucleus of HCT 116 cells. HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an Apoptosis-enhancing nuclease polyclonal antibody (Product # PA5-22208) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 236 and 325 of Apoptosis-enhancing nuclease (Uniprot ID#Q8WTP8)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22208 targets Apoptosis-enhancing nuclease in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-22208 immunogen is a synthetic peptide corresponding to a region within amino acids 236 and 325 of Apoptosis-enhancing nuclease (Uniprot ID#Q8WTP8).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.