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Aquaporin 0 Antibody (PA5-120840) in IHC (P)
Immunohistochemistry analysis of Aquaporin 0 in paraffin-embedded human stomach. Samples were incubated with Aquaporin 0 Polyclonal antibody (Product # PA5-120840) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-120840
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).
Conjugate
Form
Liquid
Concentration
0.83 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914412
Product Specific Information
Positive test controls include: HepG2, U-251MG, Mouse skeletal muscle, Mouse heart, Mouse liver, Mouse testis, Mouse eye, Rat eye. The target is usually found in the following locations: Cell junction, Cell membrane, Multi-pass membrane protein, gap junction.
Immunogen sequence: LALATLVQSV GHISGAHVNP AVTFAFLVGS QMSLLRAFCY MAAQLLGAVA GAAVLYSVTP PAVRGNLALN TLHPAVSVGQ ATTVEIFLTL QFVLCIFATY D
Target Information
AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aquaporin 0; Aquaporin-0; Aquaporin0; dominant cataract; hydropic fibers; Lens fiber major intrinsic protein; lens opacity; major intrinsic protein of eye lens fiber; MIP26; MP26
Gene Aliases: AQP0; Cat; CTRCT15; Hfi; LIM1; Lop; MIP; MIP26; MP26; Palm; shrivelled; Svl
UniProt ID: (Human) P30301, (Mouse) P51180, (Rat) P09011
Entrez Gene ID: (Human) 4284, (Mouse) 17339, (Rat) 25480
lens development in camera-type eye
water transport
visual perception
cellular water homeostasis
canalicular bile acid transport
glycerol transport
ion transmembrane transport
positive regulation of cell adhesion
response to stimulus
protein homotetramerization
gap junction-mediated intercellular transport
transport
cell communication
carbohydrate transmembrane transport
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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