Invitrogen

Aquaporin 2 Polyclonal Antibody

Product Image Gallery

Aquaporin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Aquaporin 2 in paraffin-embedded rat kidney. Samples were probed with Aquaporin 2 polyclonal antibody (Product # PA5-77707) using a dilution of 1:100, and incubated with hematoxylin.

Aquaporin 2 Antibody in Western Blot (WB) — Western blot analysisof rat kidney membranes with Aquaporin 2 polyclonal antibody (Product # PA5-77707) using a dilution of 1:200.

Aquaporin 2 Antibody — (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:200-1:1000

Western Blot (WB)

1:200-1:1000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2735463

Product Specific Information

Product is shipped at room temperature as a lyophilized powder and should be stored at -20 °C upon receipt. Reconstitution: add 50 µL of deionized water.

Target Information

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ADH water channel; AQP-2; AQP-CD; AQP2; aquaporin 2 (collecting duct); Aquaporin-2; Aquaporin-CD; Collecting duct water channel protein; Water channel protein for renal collecting duct; water-channel aquaporin 2; WCH-CD

Gene Aliases: AQP-2; AQP-CD; AQP2; aquaporin-2; cph; jpk; WCH-CD

UniProt ID: (Human) P41181, (Mouse) P56402, (Rat) P34080

Entrez Gene ID: (Human) 359, (Mouse) 11827, (Rat) 25386

Molecular Function: transporter