Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 10
Aquaporin 4 Antibody (PA5-85767) in ICC/IF
Aquaporin 4 Polyclonal Antibody detects Aquaporin 4 protein at astrocyte cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aquaporin 4 protein stained by Aquaporin 4 Polyclonal Antibody (Product # PA5-85767) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta-3 Tubulin Po... View More
Product Details
PA5-85767
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse Aquaporin 4.
Conjugate
Form
Liquid
Concentration
0.31 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792904
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Human (92%), Mouse (100%), Rat (98%), Pig (92%), Sheep (95%), Rhesus Monkey (95%), Bovine (95%).
Positive Control: mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In skeletal muscle, AQP4 (aquaporin 4 also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AQP-4; Aquaporin-4; Aquaporin4; HMIWC2; Mercurial-insensitive water channel; MGC22454; MIWC; WCH4
Gene Aliases: AQP-4; Aqp4; Miwc; MIWC2; WCH4
UniProt ID: (Mouse) P55088, (Rat) P47863
Entrez Gene ID: (Mouse) 11829, (Rat) 25293
transport
water transport
sensory perception of sound
cellular water homeostasis
regulation of vascular endothelial growth factor production
carbon dioxide transport
glycerol transport
water homeostasis
negative regulation of interleukin-1 beta production
negative regulation of interleukin-6 production
carbohydrate transmembrane transport
ion transmembrane transport
protein homooligomerization
transmembrane transport
negative regulation of cell adhesion molecule production
renal water absorption
cellular response to glucose stimulus
cellular response to interferon-gamma
female pregnancy
response to radiation
hyperosmotic salinity response
multicellular organismal water homeostasis
response to glucocorticoid
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support