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Artemin Antibody (500-P245-1MG) in WB
Western Blot: To detect Human Artemin by Western Blot analysis Artemin Polyclonal Antibody (Product # 500-P245-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Artemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P245-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Artemin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930027
Product Specific Information
AA Sequence of recombinant protein: AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Artemin. Anti-Human Artemin-specific antibody was purified by affinity chromatography employing an immobilized Human Artemin matrix.
Sandwich ELISA: To detect Human Artemin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Artemin (500-P245Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Artemin.
Western Blot: To detect Human Artemin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Artemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Artemin; Enovin; Neublastin; neurotrophic factor
Gene Aliases: ARTN; ENOVIN; EVN; NBN
UniProt ID: (Human) Q5T4W7
Entrez Gene ID: (Human) 9048
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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