Immunohistochemical analysis of Artemis in formalin-fixed, paraffin-embedded human spleen tissue using an Artemis polyclonal antibody (Product # PA1-31797) at a dilution of 1:1000 showing positive staining of T cells and B lymphocytes in thymus, lymph nodes and spleen.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide CPKDTYSDLKSRDK, corresponding to amino acids 597-610 of Human Artemis.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31797 detects Artemis from human samples.
PA1-31797 has been successfully used in Western blot applications.
The PA1-31797 immunogen is: Synthetic peptide: CPKDTYSDLKSRDK, corresponding to amino acids 597-610 of Human Artemis.
Artemis is a novel protein that is involved in V(D)J recombination/DNA repair. Mutations cause human T-B-severe combined immunodeficiency associated with increased cellular radiosensitivity (RS-SCID).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.