Immunofluorescent analysis of aryl hydrocarbon receptor (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with an aryl hydrocarbon receptor antibody (Product # PA1-515) at a dilution of 20ug/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C N(771) S Y N N E E F P D L T M F P P F S E(789)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-515 detects Ah receptor nuclear translocator (ARNT) from mouse and rat cells.
PA1-515 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~87 kDa protein representing ARNT from Hepa 1c1c7 cell lysate. PA1-515 reacts with one other band lower than ARNT whose identity has not been established.
PA1-515 immunizing peptide corresponds to amino acid residues 771-789 from human ARNT. This sequence is completely conserved between human and mouse ARNT.
The aryl hydrocarbon (Ah) receptor is a ligand activated transcription factor that binds various aryl hydrocarbons such as dioxin or benzo(a)pyrene, and mediates their toxic and carcinogenic biological responses. No endogenous ligand to the Ah receptor has been identified.
Ah receptor nuclear translocator (ARNT) is an 87 kDa basic helix/loop/helix protein that dimerizes with the Ah receptor but does not independently bind ligand. Instead, ARNT is necessary for the ligand-mediated activation of the Ah receptor within the nucleus. ARNT and Ah receptor have also been identified as members Per and Sim (PAS) protein family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Transcription factor c-Maf mediates the TGF-ß-dependent suppression of IL-22 production in T(H)17 cells.
PA1-515 was used in western blot to investigate the effect of c-Maf on the TGF-beta-dependent suppression of IL-22 production in Th17 cells
|Rutz S,Noubade R,Eidenschenk C,Ota N,Zeng W,Zheng Y,Hackney J,Ding J,Singh H,Ouyang W||Nature immunology (12:1238)||2011|
Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts.
PA1-515 was used in western blot to investigate the regulation of AhR by serum and growth factors in the cell cycle
|Vaziri C,Schneider A,Sherr DH,Faller DV||The Journal of biological chemistry (271:25921)||1996|
Cooperative E-box regulation of human GLI1 by TWIST and USF.
PA1-515 was used in EMSA to investigate the regulation of human Gli1 by TWIST and USF and its mechanism
|Villavicencio EH,Yoon JW,Frank DJ,Füchtbauer EM,Walterhouse DO,Iannaccone PM||Genesis (New York, N.Y. : 2000) (32:247)||2002|
cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt).
PA1-515 was used in immunoprecipitation to characterize Arnt2 and investigate its tissue distribution and biochemical properties
|Hirose K,Morita M,Ema M,Mimura J,Hamada H,Fujii H,Saijo Y,Gotoh O,Sogawa K,Fujii-Kuriyama Y||Molecular and cellular biology (16:1706)||1996|