Immunofluorescent analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Pig, Non-human primate, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:100 - 1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10 - 1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-514 has been successfully used in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~96 kDa protein representing AhR, with some minor non-specific bands observed in certain samples.
MA1-514 immunogen is residues 12-31 from the human AhR protein with amino acids 18-21 being omitted.
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Induction of a chloracne phenotype in an epidermal equivalent model by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on aryl hydrocarbon receptor activation and is not reproduced by aryl hydrocarbon receptor knock down.
MA1-514 was used in western blot to study the role of aryl hydrocarbon receptor activation in chloracne induction by TCDD in a human epidermal equivalent model
|Forrester AR,Elias MS,Woodward EL,Graham M,Williams FM,Reynolds NJ||Journal of dermatological science (73:10)||2014|
HER2 overexpression-mediated inflammatory signaling enhances mammosphere formation through up-regulation of aryl hydrocarbon receptor transcription.
MA1-514 was used in western blot to study the role of HER2-aryl hydrocarbon receptor interactions in mammosphere formation in MCF-7 breast cancer cells
|Zhao S,Ohara S,Kanno Y,Midorikawa Y,Nakayama M,Makimura M,Park Y,Inouye Y||Cancer letters (330:41)||2013|
Aryl hydrocarbon receptor regulates the cholesterol biosynthetic pathway in a dioxin response element-independent manner.
MA1-514 was used in western blot to investigate the role of aryl hydrocarbon receptor in cholesterol biosynthetic pathway
|Tanos R,Patel RD,Murray IA,Smith PB,Patterson AD,Perdew GH||Hepatology (Baltimore, Md.) (55:1994)||2012|
Activation of the aryl hydrocarbon receptor represses mammosphere formation in MCF-7 cells.
MA1-514 was used in western blot to study the suppression of mammosphere formation in MCF-7 cells by aryl hydrocarbon receptor activation
|Zhao S,Kanno Y,Nakayama M,Makimura M,Ohara S,Inouye Y||Cancer letters (317:192)||2012|
Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver.
MA1-514 was used in western blot to study the effects of maternal smoking and fetal sex on metabolic enzyme expression in the fetal liver
|O'Shaughnessy PJ,Monteiro A,Bhattacharya S,Fowler PA||The Journal of clinical endocrinology and metabolism (96:2851)||2011|
Suppression mechanisms of flavonoids on aryl hydrocarbon receptor-mediated signal transduction.
MA1-514 was used in western blot to investigate the mechanisms by which flavonoids suppress the AhR-mediated signal transduction in mouse hepatoma Hepa-1c1c7 cells
|Mukai R,Shirai Y,Saito N,Fukuda I,Nishiumi S,Yoshida K,Ashida H||Archives of biochemistry and biophysics (501:134)||2010|
Differential gene regulation by the human and mouse aryl hydrocarbon receptor.
MA1-514 was used in western blot to characterize mouse vs. human divergence in receptor-regulated gene expression
|Flaveny CA,Murray IA,Perdew GH||Toxicological sciences : an official journal of the Society of Toxicology (114:217)||2010|
Evidence for ligand-mediated selective modulation of aryl hydrocarbon receptor activity.
MA1-514 was used in western blot to study the regulation of Aryl hydrocarbon receptor activity and its anti-inflammatory therapeutic potential
|Murray IA,Morales JL,Flaveny CA,Dinatale BC,Chiaro C,Gowdahalli K,Amin S,Perdew GH||Molecular pharmacology (77:247)||2010|
Antagonism of aryl hydrocarbon receptor signaling by 6,2',4'-trimethoxyflavone.
MA1-514 was used in western blot to investigate the effect of 6,2',4'-trimethoxyflavone for aryl hydrocarbon receptor signaling activity
|Murray IA,Flaveny CA,DiNatale BC,Chairo CR,Schroeder JC,Kusnadi A,Perdew GH||The Journal of pharmacology and experimental therapeutics (332:135)||2010|
Omeprazole stimulates the induction of human insulin-like growth factor binding protein-1 through aryl hydrocarbon receptor activation.
MA1-514 was used in western blot to study the effect of omeprazole on IGFBP-1-dependent physiological processes.
|Murray IA,Perdew GH||The Journal of pharmacology and experimental therapeutics (324:1102)||2008|
Differentiation-specific factors modulate epidermal CYP1-4 gene expression in human skin in response to retinoic acid and classic aryl hydrocarbon receptor ligands.
MA1-514 was used in western blot to investigate the change of CYP1-4 gene expression in differentiating epidermal cells exposed to drug compounds related to clinical dermatology practice or foreign compounds affecting normal epidermal differentiation
|Du L,Neis MM,Ladd PA,Keeney DS||The Journal of pharmacology and experimental therapeutics (319:1162)||2006|
Dioxin exerts anti-estrogenic actions in a novel dioxin-responsive telomerase-immortalized epithelial cell line of the porcine oviduct (TERT-OPEC).
MA1-514 was used in western blot to study the impact of PHAHs on oviduct epithelium treated with dioxin-type endocrine disruptors
|Hombach-Klonisch S,Pocar P,Kauffold J,Klonisch T||Toxicological sciences : an official journal of the Society of Toxicology (90:519)||2006|
AhR-agonist-induced transcriptional changes of genes involved in thyroid function in primary porcine thyrocytes.
MA1-514 was used in western blot to investigate the effects of TCDD and dioxin-like PCB 127 on the AhR signaling pathway and on the gene expression profiles of key factors related to thyroid function
|Pocar P,Klonisch T,Brandsch C,Eder K,Fröhlich C,Hoang-Vu C,Hombach-Klonisch S||Toxicological sciences : an official journal of the Society of Toxicology (89:408)||2006|
Dioxin-induced immortalization of normal human keratinocytes and silencing of p53 and p16INK4a.
MA1-514 was used in western blot to study the mechanism of TCDD-induced immortalization of primary keratinocytes.
|Ray SS,Swanson HI||The Journal of biological chemistry (279:27187)||2004|
HES-1, a novel target gene for the aryl hydrocarbon receptor.
MA1-514 was used in western blot to study how HES-1 is regulated by estrogen receptor and AhR
|Thomsen JS,Kietz S,Ström A,Gustafsson JA||Molecular pharmacology (65:165)||2004|
|Non-human primate||Not Cited||
Characterization of the phosphorylation status of the hepatitis B virus X-associated protein 2.
MA1-514 was used in western blot to indentify the phosphorylation status of the hepatitis B virus X-associated protein 2
|Dull AB,Carlson DB,Petrulis JR,Perdew GH||Archives of biochemistry and biophysics (406:209)||2002|
Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization.
MA1-514 was used in western blot to investigate the function of XAP2 for the regulation of AhR stabilization
|Meyer BK,Perdew GH||Biochemistry (38:8907)||1999|
Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts.
MA1-514 was used in western blot to investigate the regulation of AhR by serum and growth factors in the cell cycle
|Vaziri C,Schneider A,Sherr DH,Faller DV||The Journal of biological chemistry (271:25921)||1996|
Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex.
MA1-514 was used in western blot to identify the subunits of AhR complex
|Chen HS,Perdew GH||The Journal of biological chemistry (269:27554)||1994|
Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch.
MA1-514 was used in ChIP assay to investigate the role of dioxin receptor in the insulator activity of B1-X35S retrotransposon
|Román AC,González-Rico FJ,Moltó E,Hernando H,Neto A,Vicente-Garcia C,Ballestar E,Gómez-Skarmeta JL,Vavrova-Anderson J,White RJ,Montoliu L,Fernández-Salguero PM||Genome research (21:422)||2011|
Genome-wide B1 retrotransposon binds the transcription factors dioxin receptor and Slug and regulates gene expression in vivo.
MA1-514 was used in ChIP assay to investigate the role of genome-wide B1 retrotransposon on regulates gene expression in vivo
|Roman AC,Benitez DA,Carvajal-Gonzalez JM,Fernandez-Salguero PM||Proceedings of the National Academy of Sciences of the United States of America (105:1632)||2008|
Inhibition of P-glycoprotein enhances the suppressive effect of kaempferol on transformation of the aryl hydrocarbon receptor.
MA1-514 was used in ELISA to investigate the role of P-glycoprotein for supression on transformation of the aryl hydrocarbon receptor by kaempferol
|Mukai R,Satsu H,Shimizu M,Ashida H||Bioscience, biotechnology, and biochemistry (73:1635)||2009|
A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists.
MA1-514 was used in ELISA to evaluate a novel ELISA strategy for the screening of aryl hydrocarbon receptor activators and inhibitors
|Fukuda I,Nishiumi S,Yabushita Y,Mukai R,Kodoi R,Hashizume K,Mizuno M,Hatanaka Y,Ashida H||Journal of immunological methods (287:187)||2004|