Immunofluorescent analysis of Aryl Hydrocarbon Receptor using Aryl Hydrocarbon Receptor Monoclonal Antibody (RPT9) (Product# MA1-513 ) shows staining in A2058 Cells. Aryl Hydrocarbon Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Aryl Hydrocarbon Receptor (Product# MA1-513 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG3, kappa|
|Immunogen||Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:100|
|Flow Cytometry (Flow)||1:50|
|Gel Shift (GS)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Gel Shift (GS)||See 3 publications below|
|ChIP assay (ChIP)||See 3 publications below|
|ELISA (ELISA)||See 2 publications below|
MA1-513 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.
MA1-513 has been successfully used in immunoprecipitation, chromatin immunoprecipitation, immunocytochemistry, immunohistochemistry, immunofluorescence, and gel shift procedures. This antibody specifically immunoprecipitates a single ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-513 can be used in Western blotting procedures, MA1-514 (clone RPT1) is recommended for this procedure.
The MA1-513 immunogen is a synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The activation mechanism of the aryl hydrocarbon receptor (AhR) by molecular chaperone HSP90.
MA1-513 was used in immunoprecipitation to study AhR and HSP90 interactions and localization
|Tsuji N,Fukuda K,Nagata Y,Okada H,Haga A,Hatakeyama S,Yoshida S,Okamoto T,Hosaka M,Sekine K,Ohtaka K,Yamamoto S,Otaka M,Grave E,Itoh H||FEBS open bio (4:796)||2014|
A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A.
MA1-513 was used in immunoprecipitation to study the polymorphisms of MFSD2A gene and their effects on its promoter activity
|Colombo F,Falvella FS,Galvan A,Frullanti E,Kunitoh H,Ushijima T,Dragani TA||Molecular cancer (10:null)||2011|
Tobacco smoke-induced skin pigmentation is mediated by the aryl hydrocarbon receptor.
MA1-513 was used in immunocytochemistry to study the role of the aryl hydrocarbon receptor in the mechanism by which tobacco smoke induces skin pigmentation
|Nakamura M,Ueda Y,Hayashi M,Kato H,Furuhashi T,Morita A||Experimental dermatology (22:556)||2013|
Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines.
MA1-513 was used in western blot to investigate the influence of TCDD exposure on human extravillous cytotrophoblast lines
|Fukushima K,Tsukimori K,Li D,Takao T,Morokuma S,Kato K,Seki H,Takeda S,Matsumura S,Wake N||Human and experimental toxicology (31:550)||2012|
Cytochromes P4501 (CYP1): catalytic activities and inducibility by diesel exhaust particle extract and benzo[a]pyrene in intact human lung ex vivo.
MA1-513 was used in western blot to investigate cytochromes P450 activity and its regulation by diesel exhaust particle extract and benzo[a]pyrene in intact human lung
|Iba MM,Shin M,Caccavale RJ||Toxicology (273:35)||2010|
2,3,7,8-tetrachlorodibenzo-p-dioxin impairs an insulin signaling pathway through the induction of tumor necrosis factor-alpha in adipocytes.
MA1-513 was used in western blot to investigate the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on insulin signaling pathway in adipocytes
|Nishiumi S,Yoshida M,Azuma T,Yoshida K,Ashida H||Toxicological sciences : an official journal of the Society of Toxicology (115:482)||2010|
Aryl hydrocarbon receptor functions as a potent coactivator of E2F1-dependent trascription activity.
MA1-513 was used in western blot to investigate the role of aryl hydrocarbon receptor in gene transcription
|Watabe Y,Nazuka N,Tezuka M,Shimba S||Biological and pharmaceutical bulletin (33:389)||2010|
7-ketocholesterol is an endogenous modulator for the arylhydrocarbon receptor.
MA1-513 was used in western blot to identify 7-ketocholesterol as an endogenous modulator that inhibits transactivation by the arylhydrocarbon receptor through competitive binding against xenobiotic ligands.
|Savouret JF,Antenos M,Quesne M,Xu J,Milgrom E,Casper RF||The Journal of biological chemistry (276:3054)||2001|
A role of the aryl hydrocarbon receptor in attenuation of colitis.
MA1-513 was used in immunohistochemistry to investigate the importance of aryl hydrocarbon receptor in intestinal immunity
|Furumatsu K,Nishiumi S,Kawano Y,Ooi M,Yoshie T,Shiomi Y,Kutsumi H,Ashida H,Fujii-Kuriyama Y,Azuma T,Yoshida M||Digestive diseases and sciences (56:2532)||2011|
Aryl hydrocarbon receptor-mediated up-regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier.
MA1-513 was used in EMSA to study the role of aryl hydrocarbon receptor in the regulation of ATP-driven xenobiotic efflux transporters at the blood-brain barrier
|Wang X,Hawkins BT,Miller DS||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:644)||2011|
2,3,7,8-Tetrachlorodibenzo-p-dioxin induces insulin-like growth factor binding protein-1 gene expression and counteracts the negative effect of insulin.
MA1-513 was used in EMSA assay to investigate the effect of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on insulin-like growth factor binding protein-1 (IGFBP-1) expression
|Marchand A,Tomkiewicz C,Marchandeau JP,Boitier E,Barouki R,Garlatti M||Molecular pharmacology (67:444)||2005|
Dietary polyphenols increase paraoxonase 1 gene expression by an aryl hydrocarbon receptor-dependent mechanism.
MA1-513 was used in EMSA to investigate the role of dietary polyphenols in paraoxonase 1 gene expression.
|Gouédard C,Barouki R,Morel Y||Molecular and cellular biology (24:5209)||2004|
Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination.
MA1-513 was used in ChIP assay to investigate the role of aryl hydrocarbon receptor in p53 ubiquitination and Ube2l3 gene expression
|Reyes-Hernández OD,Mejía-García A,Sánchez-Ocampo EM,Cabañas-Cortés MA,Ramírez P,Chávez-González L,Gonzalez FJ,Elizondo G||Biochemical pharmacology (80:932)||2010|
Aryl hydrocarbon receptor is a transcriptional activator of the human breast cancer resistance protein (BCRP/ABCG2).
MA1-513 was used in ChIP assay to study the role of aryl hydrocarbon receptors in regulation of breast cancer resistance
|Tan KP,Wang B,Yang M,Boutros PC,Macaulay J,Xu H,Chuang AI,Kosuge K,Yamamoto M,Takahashi S,Wu AM,Ross DD,Harper PA,Ito S||Molecular pharmacology (78:175)||2010|
Role of the coiled-coil coactivator (CoCoA) in aryl hydrocarbon receptor-mediated transcription.
MA1-513 was used in chromatin immunoprecipitation to investigate the role of the coiled-coil coactivator (CoCoA) during the AHR/ARNT-mediated transcription.
|Kim JH,Stallcup MR||The Journal of biological chemistry (279:49842)||2004|
A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists.
MA1-513 was used in ELISA to evaluate a novel ELISA strategy for the screening of aryl hydrocarbon receptor activators and inhibitors
|Fukuda I,Nishiumi S,Yabushita Y,Mukai R,Kodoi R,Hashizume K,Mizuno M,Hatanaka Y,Ashida H||Journal of immunological methods (287:187)||2004|
Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization.
MA1-513 was used in ELISA to investigate the function of XAP2 for the regulation of AhR stabilization
|Meyer BK,Perdew GH||Biochemistry (38:8907)||1999|