|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Crude rat liver membrane extracts|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-40244 detects ASGPR from rat and human samples.
MA1-40244 has been successfully used in ELISA, flow cytometry, immunohistochemistry (frozen), immunofluorescence, and Western blot applications. The typical starting working dilution is 1:10.
The MA1-40244 immunogen is crude rat liver membrane extracts.
Positive control: Rat hepatocytes
Negative control: Rat kidney, pancreas, small intestine, colon
The asialoglycooprotein receptor (ASGPR) is a transmembrane glycoprotein (42 kDa) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral palasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediate the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed.The rat asialoglycoprotein receptor (ASGPR) consists of three polypeptide subunits: rat hepatic lectin (RHL)-1, RHL-2 and RHL-3. The monoclonal antibody 8D7 recognizes a subunit-specific epitope of RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture.
MA1-40244 was used in immunocytochemistry to evaluate a culture method for the maturation of induced pluripotent stem cell derived hepatocytes
|Gieseck RL,Hannan NR,Bort R,Hanley NA,Drake RA,Cameron GW,Wynn TA,Vallier L||PloS one (9:null)||2014|