Immunofluorescence analysis of Aurora A Antibody (35C1)was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora A Antibody (35C1)(458900) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic and Nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Bovine, Dog, Horse, Human, Mouse, Non-human primate, Pig, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein derived from the full length of human Aurora A Kinase protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
14-3-3¿ Prevents Centrosome Amplification and Neoplastic Progression.
45-8900 was used in western blot to analyze prevention of centrosome amplification and neoplastic progression by 14-3-3gamma
|Mukhopadhyay A,Sehgal L,Bose A,Gulvady A,Senapati P,Thorat R,Basu S,Bhatt K,Hosing AS,Balyan R,Borde L,Kundu TK,Dalal SN||Scientific reports (6:null)||2016|
Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis.
45-8900 was used in western blot to examine how histone H3 threonine 118 alters chromosome structure
|Wike CL,Graves HK,Hawkins R,Gibson MD,Ferdinand MB,Zhang T,Chen Z,Hudson DF,Ottesen JJ,Poirier MG,Schumacher J,Tyler JK||eLife (5:null)||2016|