|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresopnding to the region encoded by exons 5 and 6 of human Aurora A.|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunoprecipitation (IP)||1-5 µg/mg of lysate|
|Western Blot (WB)||0.05-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25579 detects Aurora A from human samples.
PA1-25579 has been successfully used in immunoprecipitation, and Western blot procedures.
The PA1-25579 immunogen is a synthetic peptide corresopnding to the region encoded by exons 5 and 6 of human Aurora A.
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.