Invitrogen

Aurora A Monoclonal Antibody (35C1)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (44) Aurora A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Aurora A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Aurora A Antibody (45-8900) in ICC/IF

Immunofluorescent analysis of Aurora A Antibody (35C1)was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora A Antibody (35C1) (Product # 45-8900) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 mi... View More

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Product Details

45-8900

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

5 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Human, Mouse, Non-human primate, Pig, Rat

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

35C1

Immunogen

Recombinant protein derived from the full length of human Aurora A Kinase protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533839

Target Information

AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Aurora Kinase Inhibitor TAK901 Inhibits Glioblastoma Growth by Blocking SREBP1-Mediated Lipid Metabolism.

Cancers

Zhan X,Qiu R,He Y,Zhao Z,Huang M,Liu Q,Zhi F,Long W

45-8900 was used in Western Blotting to provide evidence that TAK901 inhibits GBM growth by suppressing SREBP1-mediated lipid metabolism.

Fri Nov 25 00:00:00 EST 2022

iScience

Speight P,Rozycki M,Venugopal S,Szászi K,Kofler M,Kapus A

45-8900 was used in Western Blot to show that (1) both MRTF and SRF are indispensable for serum-induced PC resorption, and (2) they act via both transcriptional and local mechanisms.

Fri Jul 23 00:00:00 EDT 2021

iScience

Speight P,Rozycki M,Venugopal S,Szászi K,Kofler M,Kapus A

45-8900 was used in Western Blot to show that (1) both MRTF and SRF are indispensable for serum-induced PC resorption, and (2) they act via both transcriptional and local mechanisms.

Fri Jul 23 00:00:00 EDT 2021

Targeting Aurora A Kinase (AAK) in Platinum-Resistant High Grade Serous Ovarian Cancer.

Frontiers in oncology

Ganapathi RN,Norris EJ,Sutker AP,Klotz KE,Ganapathi MK

45-8900 was used in Western Blotting to show that in HGSOC without BRCA1/2 mutations, CP, or PT resistance can potentially be circumvented by sequential treatment with AL that inhibits AAK involved in G2-M transition.

Mon Sep 28 00:00:00 EDT 2020

Targeting Aurora A Kinase (AAK) in Platinum-Resistant High Grade Serous Ovarian Cancer.

Frontiers in oncology

Ganapathi RN,Norris EJ,Sutker AP,Klotz KE,Ganapathi MK

Mon Sep 28 00:00:00 EDT 2020

A novel mitosis-specific Cep215 domain interacts with Cep192 and phosphorylated Aurora A for organization of spindle poles.

Journal of cell science

Kuriyama R,Fisher CR

45-8900 was used in Immunocytochemistry to conclude that Cep215 plays a role in maintaining the structural integrity of the spindle pole by providing a platform for the molecules involved in centrosome maturation.

Tue Dec 29 00:00:00 EST 2020

Quantitative Temporal Viromics of an Inducible HIV-1 Model Yields Insight to Global Host Targets and Phospho-Dynamics Associated with Protein Vpr.

Molecular & cellular proteomics : MCP

Lapek JD,Lewinski MK,Wozniak JM,Guatelli J,Gonzalez DJ

45-8900 was used in Western Blotting to define mechanisms of HIV viral protein action by identifying host proteins whose abundance is affected by Vpr, and the corresponding modulation of down-stream signalling pathways, specifically those regulated by phosphorylation.

Tue Aug 01 00:00:00 EDT 2017

Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis.

eLife

Wike CL,Graves HK,Hawkins R,Gibson MD,Ferdinand MB,Zhang T,Chen Z,Hudson DF,Ottesen JJ,Poirier MG,Schumacher J,Tyler JK

45-8900 was used in western blot to examine how histone H3 threonine 118 alters chromosome structure

Tue Feb 16 00:00:00 EST 2016

14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression.

Scientific reports

Mukhopadhyay A,Sehgal L,Bose A,Gulvady A,Senapati P,Thorat R,Basu S,Bhatt K,Hosing AS,Balyan R,Borde L,Kundu TK,Dalal SN

45-8900 was used in western blot to analyze prevention of centrosome amplification and neoplastic progression by 14-3-3gamma

Thu Jun 02 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: ARK-1; AURAK; Aurora 2; aurora A; Aurora A Kinase; aurora family kinase 1; Aurora kinase A; Aurora-A; aurora-related kinase 1; aurora/IP; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; EC 2.7.11.1; hARK1; Ipl1- and aurora-related kinase 1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; ratAurA; RP5-1167H4.6; sereine threonine kinase; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1

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Gene Aliases: AIK; AIRK1; ARK-1; ARK1; AU019385; AURA; AURKA; Aurora-A; AW539821; AYK1; BTAK; IAK; IAK1; PPP1R47; STK15; STK6; STK7

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UniProt ID: (Bovine) Q2TA06, (Pig) A5GFW1, (Human) O14965, (Mouse) P97477

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Entrez Gene ID: (Dog) 485940, (Horse) 100050161, (Bovine) 504437, (Pig) 574063, (Human) 6790, (Mouse) 20878, (Rat) 261730

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Function(s)

protein kinase activity ATP binding protein kinase binding ubiquitin protein ligase binding protein heterodimerization activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding histone serine kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

spindle assembly involved in female meiosis I mitotic centrosome separation response to wounding anterior/posterior axis specification negative regulation of gene expression regulation of protein stability negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process histone-serine phosphorylation negative regulation of apoptotic process cell division centrosome localization protein localization to centrosome liver regeneration positive regulation of oocyte maturation neuron projection extension meiotic spindle organization cell projection organization positive regulation of translation positive regulation of meiotic cell cycle process involved in oocyte maturation G2/M transition of mitotic cell cycle protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest spindle organization mitotic spindle organization mitotic nuclear division anaphase-promoting complex-dependent catabolic process response to estradiol regulation of cytokinesis protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of mitotic nuclear division regulation of centrosome cycle protein autophosphorylation regulation of signal transduction by p53 class mediator microtubule cytoskeleton organization mitotic cell cycle cell cycle meiotic nuclear division phosphorylation centrosome organization negative regulation of spindle checkpoint

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Aurora A Monoclonal Antibody (35C1)

Aurora A Antibody (45-8900) in ICC/IF

Product Details

45-8900

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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