|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant Aurora A|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 - 5µg|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1/500-1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Clone 35C1 specifically recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A, and does not inhibit Aurora-A kinase activity.
A suggested positive control for immunohistochemical applications is human 293 and mouse llc1 cell lines. In Western blot, this antibody detects a band at ~46kDa in human HeLa and mouse M-ICc12 cell lysates.
Aurora-A kinase is a 46kDa serine/threonine kinase involved in mitotic spindle assembly. Aurora A is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumourigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.