|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein purified from E.coli (His-Aurora B)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is A431 cells.
Aurora protein kinase family (consisting of Aurora-A,-B and-C) is an important group of enzymes that controls several aspects of cell division in mammalian cells. In humans, Aurora-A is localized to the centrosome in prophase, subsequently spreading to the mitotic spindles/centrosomes. Aurora-B is found at the kinetochore, mainly at the midzone, during anaphase. Aurora-C is localized to the centrosomes from anaphase to cytokinesis. Aurora B is the catalytic component of the chromosomal passenger complex, which is composed of three additional noncatalytic subunits that direct its activity: survivin, INCEP, and borealin. Aurora B appears to be required for several mitotic processes, including aberrant chromosome alignment/segregation and cytokinesis. Phosphorylation of Histone H3 (Ser10 and Ser28) by Aurora B contributes to chromosome number instability and mitotic chromosome condensation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.