Immunofluorescence analysis of Aurora C was performed using 70% confluent log phase HeLa cells treated with 3 uM of Nocodazole for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Aurora C Rabbit Polyclonal Antibody (389400) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows untreated cells with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the human Aurora C (Serine/threonine-protein kinase 13, Serine/threonine kinase AIE2, Aurora/IPL1/Eg2 protein 2, Aurora/IPL1-related kinase 3) protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex.
38-9400 was used in immunoprecipitation and western blot to characterize shared and distinct features compared with aurora-B chromosome passenger protein complex by Aurora-C interactions with INCENP and survivin
|Sasai K,Katayama H,Hawke DH,Sen S||PloS one (11:null)||2016|
||Aurora-B regulates RNA methyltransferase NSUN2.||Sakita-Suto S,Kanda A,Suzuki F,Sato S,Takata T,Tatsuka M||Molecular biology of the cell (18:1107)||2007|
Overexpression of active Aurora-C kinase results in cell transformation and tumour formation.
38-9400 was used in western blot to study the contribution of Aurora-A , -B, and -C to cancer.
|Khan J,Ezan F,Crémet JY,Fautrel A,Gilot D,Lambert M,Benaud C,Troadec MB,Prigent C||PloS one (6:null)||2011|
|Human||Not Cited||Aurora-B regulates RNA methyltransferase NSUN2.||Sakita-Suto S,Kanda A,Suzuki F,Sato S,Takata T,Tatsuka M||Molecular biology of the cell (18:1107)||2007|
|Human||Not Cited||Anaplastic thyroid carcinoma: expression profile of targets for therapy offers new insights for disease treatment.||Wiseman SM,Masoudi H,Niblock P,Turbin D,Rajput A,Hay J,Bugis S,Filipenko D,Huntsman D,Gilks B||Annals of surgical oncology (14:719)||2007|