Invitrogen

Axl Monoclonal Antibody (DS7HAXL), PE, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (32) AXL antibodies

Datasheet

Datasheet

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Axl Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were stained with HLA-DR Monoclonal Antibody, FITC (Product # 11-9956-42), CD1c Monoclonal Antibody, APC (Product # 17-0015-42), CD11c Monoclonal Antibody, eFluor 450 (Product # 49-0116-42), and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or Axl Monoclonal Antibody, PE (right). CD11c+/CD14- cells were used for analysis.

Axl Antibody in Relative expression — Staining of human peripheral blood mononuclear cells. As expected based on known expression patterns, Axl clone DS7HAXL stains a subset of CD11c+/CD14- dendritic cells. Details: Normal human PBMC were surface stained with Axl clone DS7HAXL and co-stained with CD14, CD11c, CD1c and HLA-DR. CD11c+/CD14- cells were used for analysis. {RE}

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS7HAXL

Conjugate

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA, 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2723961

Product Specific Information

This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis.

Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

Axl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and auto-phosphorylation upon ligand binding. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (through SOCS-1 and SOCS-3), which limits TLR and cytokine signaling. This results in dampened inflammatory responses in macrophages and DCs. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ARK; AXL; AXL oncogene; AXL transforming sequence/gene; JTK11; Tyro7; Tyrosine-protein kinase receptor UFO; UFO

Gene Aliases: ARK; AXL; JTK11; Tyro7; UFO

UniProt ID: (Human) P30530

Entrez Gene ID: (Human) 558

Function(s)

neuron migration natural killer cell differentiation positive regulation of cytokine-mediated signaling pathway blood vessel remodeling phagocytosis inflammatory response signal transduction spermatogenesis peptidyl-tyrosine phosphorylation forebrain cell migration platelet activation cellular response to extracellular stimulus negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation secretion by cell erythrocyte homeostasis substrate adhesion-dependent cell spreading cellular response to interferon-alpha ovulation cycle apoptotic cell clearance protein kinase B signaling negative regulation of neuron apoptotic process innate immune response viral entry into host cell vascular endothelial growth factor receptor signaling pathway cell maturation positive regulation of pinocytosis negative regulation of lymphocyte activation positive regulation of protein kinase B signaling vagina development cellular response to lipopolysaccharide dendritic cell differentiation negative regulation of dendritic cell apoptotic process

Process(es)

virus receptor activity phosphatidylserine binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding protein heterodimerization activity

Axl Monoclonal Antibody (DS7HAXL), PE, eBioscience™

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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