Invitrogen

Axl Monoclonal Antibody (DS7HAXL), PE, eBioscience™

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Axl Antibody (12-1087-42) in Flow

Knockout of AXL was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR932417_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of AXL was performed by staining U-87 MG AXL Knock out cells with0.06 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE,eBioscience™ (Product # 12-4714-82, yellow histogram) or0.06 µgAxl Monoclonal Antibody (DS7HAXL), PE, eBioscience™ (Product # 12-1087-42, blue histogram).U87MG Cas9 control cells were also stained with0.0.06 ... View More

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Axl Antibody — Staining of human peripheral blood mononuclear cells. As expected based on known expression patterns, Axl clone DS7HAXL stains a subset of CD11c+/CD14- dendritic cells. Details: Normal human PBMC were surface stained with Axl clone DS7HAXL and co-stained with CD14, CD11c, CD1c and HLA-DR. CD11c+/CD14- cells were used for analysis. {RE}

Product Details

12-1087-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS7HAXL

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2723961

Product Specific Information

This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis.

Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Oral Epithelial Cells Expressing Low or Undetectable Levels of Human Angiotensin-Converting Enzyme 2 Are Susceptible to SARS-CoV-2 Virus Infection In Vitro.

Pathogens (Basel, Switzerland)

Ebraham L,Xu C,Wang A,Hernandez C,Siclari N,Rajah D,Walter L,Marras SAE,Tyagi S,Fine DH,Daep CA,Chang TL

Published figure using Axl monoclonal antibody (Product # 12-1087-42) in Flow Cytometry

Mon Jun 19 00:00:00 UTC 2023

Single-cell profiling of alveolar rhabdomyosarcoma reveals RAS pathway inhibitors as cell-fate hijackers with therapeutic relevance.

Science advances

Danielli SG,Porpiglia E,De Micheli AJ,Navarro N,Zellinger MJ,Bechtold I,Kisele S,Volken L,Marques JG,Kasper S,Bode PK,Henssen AG,Gürgen D,Delattre O,Surdez D,Roma J,Bühlmann P,Blau HM,Wachtel M,Schäfer BW

Published figure using Axl monoclonal antibody (Product # 12-1087-42) in Flow Cytometry

Fri Feb 10 00:00:00 UTC 2023

Remodelling of tumour microenvironment by microwave ablation potentiates immunotherapy of AXL-specific CAR T cells against non-small cell lung cancer.

Nature communications

Cao B,Liu M,Wang L,Zhu K,Cai M,Chen X,Feng Y,Yang S,Fu S,Zhi C,Ye X,Zhang J,Zhang Z,Yang X,Zhao M,Wu Q,Xu L,Yang L,Lian H,Zhao Q,Zhang Z

Published figure using Axl monoclonal antibody (Product # 12-1087-42) in Flow Cytometry

Wed Oct 19 00:00:00 UTC 2022

Human Neutrophils Present Mild Activation by Zika Virus But Reduce the Infection of Susceptible Cells.

Frontiers in immunology

Aggio JB,Porto BN,Duarte Dos Santos CN,Mosimann ALP,Wowk PF

12-1087-42 was used in Flow cytometry/Cell sorting to suggest that human neutrophils do not play an antiviral role against ZIKV per se, but these cells might participate in an infected environment shaping the ZIKV infection in other target cells.

Mon Jun 27 00:00:00 UTC 2022

A Genome-Wide Haploid Genetic Screen Identifies Heparan Sulfate-Associated Genes and the Macropinocytosis Modulator TMED10 as Factors Supporting Vaccinia Virus Infection.

Journal of virology

Luteijn RD,van Diemen F,Blomen VA,Boer IGJ,Manikam Sadasivam S,van Kuppevelt TH,Drexler I,Brummelkamp TR,Lebbink RJ,Wiertz EJ

12-1087 was used in Flow cytometry/Cell sorting to show that TMED10, previously not implicated in virus infections, facilitates virus uptake by modulating the cellular response to phosphatidylserine.

Mon Jul 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO

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Gene Aliases: ARK; AXL; JTK11; Tyro7; UFO

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UniProt ID: (Human) P30530

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Entrez Gene ID: (Human) 558

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Function(s)

virus receptor activity phosphatidylserine binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding protein heterodimerization activity transmembrane signal receptor

Process(es)

neuron migration natural killer cell differentiation positive regulation of cytokine-mediated signaling pathway blood vessel remodeling phagocytosis inflammatory response signal transduction spermatogenesis peptidyl-tyrosine phosphorylation forebrain cell migration platelet activation cellular response to extracellular stimulus negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation secretion by cell erythrocyte homeostasis substrate adhesion-dependent cell spreading cellular response to interferon-alpha ovulation cycle apoptotic cell clearance protein kinase B signaling negative regulation of neuron apoptotic process innate immune response viral entry into host cell vascular endothelial growth factor receptor signaling pathway cell maturation positive regulation of pinocytosis negative regulation of lymphocyte activation positive regulation of protein kinase B signaling vagina development cellular response to lipopolysaccharide dendritic cell differentiation negative regulation of dendritic cell apoptotic process

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Axl Monoclonal Antibody (DS7HAXL), PE, eBioscience™

Axl Antibody (12-1087-42) in Flow

Product Details

12-1087-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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