Invitrogen

Axl Monoclonal Antibody (DS7HAXL), PE, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (56) AXL antibodies

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Axl Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were stained with HLA-DR Monoclonal Antibody, FITC (Product # 11-9956-42), CD1c Monoclonal Antibody, APC (Product # 17-0015-42), CD11c Monoclonal Antibody, eFluor 450 (Product # 49-0116-42), and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or Axl Monoclonal Antibody, PE (right). CD11c+/CD14- cells were used for analysis.

Axl Antibody — Staining of human peripheral blood mononuclear cells. As expected based on known expression patterns, Axl clone DS7HAXL stains a subset of CD11c+/CD14- dendritic cells. Details: Normal human PBMC were surface stained with Axl clone DS7HAXL and co-stained with CD14, CD11c, CD1c and HLA-DR. CD11c+/CD14- cells were used for analysis. {RE}

Product Details

12-1087-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.06 µg)/test	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	DS7HAXL
Conjugate	PE APC PE-Cyanine7 Super Bright 436
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA, 0.1% gelatin
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2723961

Product Specific Information

This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis.

Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

Axl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and auto-phosphorylation upon ligand binding. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (through SOCS-1 and SOCS-3), which limits TLR and cytokine signaling. This results in dampened inflammatory responses in macrophages and DCs. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A Genome-Wide Haploid Genetic Screen Identifies Heparan Sulfate-Associated Genes and the Macropinocytosis Modulator TMED10 as Factors Supporting Vaccinia Virus Infection.

Journal of virology

Luteijn RD,van Diemen F,Blomen VA,Boer IGJ,Manikam Sadasivam S,van Kuppevelt TH,Drexler I,Brummelkamp TR,Lebbink RJ,Wiertz EJ

12-1087 was used in Flow cytometry/Cell sorting to show that TMED10, previously not implicated in virus infections, facilitates virus uptake by modulating the cellular response to phosphatidylserine.