|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. A suggested positive control for immunohistochemical applications is tonsil or breast carcinoma. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
B7H4 belongs to the B7 family (see CD80; MIM 112203) of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands (e.g., CD28; MIM 186760) on T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.