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Invitrogen
BAF53A Recombinant Rabbit Monoclonal Antibody (5H3L6)
FIGURE: 1 / 2
BAF53A Antibody (702414) in ICC/IF
For immunofluorescence analysis, MDA-MB-231 cells were fixed and permeabilized for detection of endogenous BAF53A using Anti-BAF53A recombinant rabbit monoclonall Antibody (Product # 702414, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of BAF53A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Produc... View More
Product Details
702414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
5H3L6
Immunogen
Peptide corresponding to human BAF53A (aa124-142)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2665307
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 53 kDa BRG1-associated factor A; actin-like 6; actin-like 6A; actin-like 6B; Actin-like protein 6A; actin-related protein 4; Actin-related protein Baf53a; ACTL6B; ArpNbeta; BAF complex 53 kDa subunit; BAF53; BAF53A; BAF53B; BRG1-associated factor 53A; BRG1/brm-associated factor 53A; hArpN alpha; hArpN beta; INO80 complex subunit K; MGC5382; MGC5382 Arp4
Gene Aliases: 2810432C06Rik; ACTL6; ACTL6A; AI851094; ARP4; ARPN-BETA; BAF53; BAF53A; C79802; INO80K; RGD1307747
UniProt ID: (Human) O96019, (Mouse) Q9Z2N8
Entrez Gene ID: (Human) 86, (Mouse) 56456, (Rat) 361925
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin binding
transcription coactivator activity
protein binding
nucleosomal DNA binding
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
neural retina development
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
spinal cord development
regulation of growth
ATP-dependent chromatin remodeling
histone H4 acetylation
histone H2A acetylation
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
nervous system development
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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