|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 254-269 of human BLyS.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: HL-60 whole cell lysate, antigen standard for TNFSF13B (transient overexpression lysate), mouse spleen protein, mouse testis protein, mouse testis, thymus, or spleen, or HL-60, mouse thymus protein.
Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases. Secreted BLyS/BAFF/THANK acts as a potent B cell growth factor. This protein is expressed abundantly in peripheral blood lymphocytes, specifically in monocytes and macrophages. Its regulation is upregulated by interferon gamma. Overexpression of BLyS/BAFF in transgenic mice lead to increased numbers of mature B and effector T cells. These mice also develop autoimmune –like symptoms, such as, high levels of rheumatoid factors, anti-DNA autoantibodies, etc. (2). Recently, two receptors for BLyS/BAFF have been identified and termed as BCMA and TACI (1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.