Immunofluorescent analysis of BAP31 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BAP31 monoclonal antibody (Product # MA3-002) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Hamster, Human, Mouse, Non-human primate|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Amino acid residues 230-246 of BAP31 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (IHC)||1:1,000 - 1:10,000|
|Immunohistochemistry (Frozen) (IHC (F))||1/1000 - 1/10000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,500 - 1:6,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-002 detects BAP31 from human, non-human primate, bovine, mouse and hamster samples.
MA3-002 has been successfully used in Western blot, ELISA, immunoprecipitation, immunofluorescent, and immunohistochemical procedures. By Western blot, this antibody detects a 28-kDa protein corresponding to human BAP31.
The MA3-002 antigen is solubilized protein from human neuroendocrine cell line IMR-32 corresponding to the C-terminal region residues 230-246 of BAP31 protein. This sequence is conserved in non-human primate, bovine and hamster species.
BAP31 (B-Cell Receptor Associated Protein, 31-kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo- and hetero-oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL-2/BCL-XL-associated protein. In the C-terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.
MA3-002 was used in immunocytochemistry to discover that Hsp105 cooperates with Hsc70 to facilitate virus entry
|Ravindran MS,Bagchi P,Inoue T,Tsai B||PLoS pathogens (11:null)||2015|
MUC1 regulates nuclear localization and function of the epidermal growth factor receptor.
MA3-002 was used in western blot to investigate the regulation of EGFR by MUC1
|Bitler BG,Goverdhan A,Schroeder JA||Journal of cell science (123:1716)||2010|
BAP31 is involved in the retention of cytochrome P450 2C2 in the endoplasmic reticulum.
MA3-002 was used in immunoprecipitation to demonstrate the interaction between BAP31 and P450 2C2 in the ER of transfected cells and its effect on the subcellular localization and accumulation of P450.
|Szczesna-Skorupa E,Kemper B||The Journal of biological chemistry (281:4142)||2006|