|Tested species reactivity||Bovine, Hamster, Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Amino acid residues 123-229 of human BAP31.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/100|
|Immunofluorescence (IF)||1:200 - 1:6,400|
|Immunohistochemistry (IHC)||1:200 - 1:6,400|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1,500 - 1:6,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA3-003 detects BAP31 from human, non-human primate, bovine and hamster samples.
MA3-003 has been successfully used in Western blot, immunofluorescent, and immunohistochemical procedures. By Western blot, this antibody detects a 28-kDa protein corresponding to human BAP31.
The MA3-003 antigen is solubilized protein from human neuroendocrine cell line IMR-32, followed by an extract of the small lung human carcinoma cell line SCC-9. MA3-003 binds to a more proximal region of BAP31 (amino acids 123-229). This sequence is conserved in non-human primate, bovine and hamster species.
BAP31 (B-Cell Receptor Associated Protein, 31-kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo- and hetero-oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL-2/BCL-XL-associated protein. In the C-terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils.
MA3-003 was used in immunocytochemistry to investigate the interaction between BAP31 and CD11b/CD18
|Zen K,Utech M,Liu Y,Soto I,Nusrat A,Parkos CA||The Journal of biological chemistry (279:44924)||2004|