|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide near the N-terminus of human BCL-10 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
BCL-10 is a cytoplasmic protein expressed at different levels in most normal human cells and translocates into the nucleus of MALT lymphoma, especially in those with translocation (1;14)(p22;q32). BCL-10 and MALT-1 protein form a complex in MALT lymphoma. They are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenic process that leads to malignancy. BCL-10 contains a caspase- recruiting (CARD) domain and is involved in activation of caspase-9 to induce apoptosis. Mutations in BCL-10 are observed in some B and T cell lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.