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Immunofluorescent analysis of BCL-2 alpha (green) showing staining in the nucleus and cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BCL-2 alpha monoclonal antibody (Product # MA5-11757) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11757 targets BCL-2 in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.
The MA5-11757 immunogen is a synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Expression of Bcl-2 inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2, whereas the normal or hyperplastic germinal centers are negative.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of Cox-2 and Bcl-2 in Paget's disease of the breast.
MA5-11757 was used in immunohistochemistry - paraffin section to examine Cox-2 and bcl-2 expression in the development of Paget's disease
|Alikanoglu AS,Yildirim M,Suren D,Tutus B,Kaya V,Topal CS,Keser S,Karadayi AN,Kapucuoglu FN,Ayva S,Gunduz S||Asian Pacific journal of cancer prevention : APJCP (16:1041)||2015|
The relation of beclin 1 and bcl-2 expressions in high grade prostatic intraepithelial neoplasia and prostate adenocarcinoma: a tissue microarray study.
MA5-11757 was used in immunohistochemistry - paraffin section to assess beclin 1 and bcl-2 expression and investigate their relationship with clinicopathological parameters of prostate cancer and high grade prostatic intraepithelial neoplasia
|Baspinar S,Bircan S,Orhan H,Kapucuoglu N,Bozkurt KK||Pathology, research and practice (210:412)||2014|
New diagnostic markers in salivary gland tumors.
MA5-11757 was used in immunohistochemistry to study the expresion of tumor antigens in order to identify novel diagnostic markers of parotid gland tumors
|Schneider S,Kloimstein P,Pammer J,Brannath W,Grasl MCh,Erovic BM||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (271:1999)||2014|
Tissue biomarkers in prognostication of serous ovarian cancer following neoadjuvant chemotherapy.
MA5-11757 was used in immunohistochemistry to attempt to identify prognostic biomarkers in serous ovarian cancer patients treated with neoadjuvant chemotherapy
|Khandakar B,Mathur SR,Kumar L,Kumar S,Datta Gupta S,Iyer VK,Kalaivani M||BioMed research international (2014:null)||2014|
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
MA5-11757 was used in immunohistochemistry to study the prognostic value of immunohistochemical and FISH analysis of BCL2, BCL6, IGH, TP53, and MYC in Turkish patients with diffuse large B-cell lymphoma
|Akay OM,Aras BD,Isiksoy S,Toprak C,Mutlu FS,Artan S,Oner U,Gulbas Z||Cancer genetics (207:87)||2014|
The protective effects of carnosine in alcohol-induced hepatic injury in rats.
MA5-11757 was used in immunohistochemistry to study the mechanisms underlying the protective effects of carnosine in a rat model of alcohol-induced liver damage
|Baykara B,Micili SC,Tugyan K,Tekmen I,Bagriyanik H,Sonmez U,Sonmez A,Oktay G,Yener N,Ozbal S||Toxicology and industrial health (30:25)||2014|
Apoptosis through regulation of Bcl-2, Bax and Mcl-1 expressions in endometriotic cyst lesions and the endometrium of women with moderate to severe endometriosis.
MA5-11757 was used in immunohistochemistry to study the role of apoptosis in endometriosis pathophysiology by examining endometrionic cyst lesions and patient endometrium for the expression of Bcl-2, Bax and Mcl-1
|Korkmaz D,Bastu E,Dural O,Yasa C,Yavuz E,Buyru F||Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology (33:725)||2013|
Effect of blueberry pretreatment on diethylnitrosamine-induced oxidative stress and liver injury in rats.
MA5-11757 was used in immunohistochemistry to study the mechanisms underlying the beneficial effects of pre-treatment with bluberries on diethylnitrosamine-induced liver injury in rats
|Bingül İ,Başaran-Küçükgergin C,Tekkeşin MS,Olgaç V,Doğru-Abbasoğlu S,Uysal M||Environmental toxicology and pharmacology (36:529)||2013|
The effects of ulipristal on Bax/Bcl-2, cytochrome c, Ki-67 and cyclooxygenase-2 expression in a rat model with surgically induced endometriosis.
MA5-11757 was used in immunohistochemistry to use a rat model of endometriosis to study the effects of ulipristal on endometrial histology and the expression of Bax/Bcl-2, cytochrome c, Ki-67 and COX-2
|Huniadi CA,Pop OL,Antal TA,Stamatian F||European journal of obstetrics, gynecology, and reproductive biology (169:360)||2013|
Identification of novel target proteins in sebaceous gland carcinoma.
MA5-11757 was used in immunohistochemistry to identify novel therapeutic target proteins in sebaceous gland carcinoma
|Erovic BM,Al Habeeb A,Harris L,Goldstein DP,Kim D,Ghazarian D,Irish JC||Head & neck (35:642)||2013|
Association of RASSF1A and p63 with poor recurrence-free survival in node-negative stage I-II non-small cell lung cancer.
MA5-11757 was used in immunohistochemistry to study the value of RASSF1A and p63 alterations in predicting node-negative stage I-II non-small cell lung cancer recurrence-free survival
|Ko E,Lee BB,Kim Y,Lee EJ,Cho EY,Han J,Shim YM,Park J,Kim DH||Clinical cancer research : an official journal of the American Association for Cancer Research (19:1204)||2013|
Biomarkers of parathyroid carcinoma.
MA5-11757 was used in immunohistochemistry to establish a biomarker panel suitable for the identification of parathyroid carcinomas
|Erovic BM,Harris L,Jamali M,Goldstein DP,Irish JC,Asa SL,Mete O||Endocrine pathology (23:221)||2012|
Resveratrol attenuates doxorubicin-induced cellular damage by modulating nitric oxide and apoptosis.
MA5-11757 was used in immunohistochemistry to study the mechanism by which resveratrol attenuates doxorubicin-induced cellular damage
|Oktem G,Uysal A,Oral O,Sezer ED,Olukman M,Erol A,Akgur SA,Bilir A||Experimental and toxicologic pathology : official journal of the Gesellschaft fu¿r Toxikologische Pathologie (64:471)||2012|
Prognostic value of oncoprotein expressions in thyroid papillary carcinoma.
MA5-11757 was used in immunohistochemistry to identify the relationship between oncoprotein expression and clinical symptoms of carcinoma
|Balta AZ,Filiz AI,Kurt Y,Sucullu I,Yucel E,Akin ML||Medical oncology (Northwood, London, England) (29:734)||2012|
Pericardial synovial sarcoma, a potential for misdiagnosis: clinicopathologic and molecular cytogenetic analysis of three cases with literature review.
MA5-11757 was used in immunohistochemistry to report on three cases of pericardial synovial sarcoma
|Cheng Y,Sheng W,Zhou X,Wang J||American journal of clinical pathology (137:142)||2012|
A unique case of follicular lymphoma provides insights to the clonal evolution from follicular lymphoma in situ to manifest follicular lymphoma.
MA5-11757 was used in immunohistochemistry to investigate the progression of follicular lymphoma in situ
|Bonzheim I,Salaverria I,Haake A,Gastl G,Adam P,Siebert R,Fend F,Quintanilla-Martinez L||Blood (118:3442)||2011|
P53, bcl-2, and nm23 expressions in serous ovarian tumors: correlation with the clinical and histopathological parameters.
MA5-11757 was used in immunohistochemistry to study the diagnostic value of p53, bcl-2 and nm23 in serous ovarian tumors
|Arik D,Kulaçoğlu S||Tu¿rk patoloji dergisi (27:38)||2011|
Heme oxygenase-1 prevents hyperthyroidism induced hepatic damage via an antioxidant and antiapoptotic pathway.
MA5-11757 was used in immunohistochemistry to investigate the protective effect of heme oxygenase-1 against hyperthyroidism and its mechanism
|Giriş M,Erbil Y,Depboylu B,Mete O,Türkoğlu U,Abbasoğlu SD,Uysal M||The Journal of surgical research (164:266)||2010|
Increased expression of CCAAT/enhancer-binding protein beta in proliferative inflammatory atrophy of the prostate: relation with the expression of COX-2, the androgen receptor, and presence of focal chronic inflammation.
MA5-11757 was used in immunohistochemistry to study the mechanisms driving increased expression of C/EBPbeta in proliferative inflammatory atrophy of the prostate
|Wang W,Bergh A,Damber JE||The Prostate (67:1238)||2007|
The correlation between the immunostains for p53 and Ki67 with bcl-2 expression and classical prognostic factors in colorectal carcinomas.
MA5-11757 was used in immunohistochemistry to study p53 and Ki67 status and their relationship with bcl-2 expression and classical prognostic factors in colorectal carcinoma
|Gurzu S,Jung J,Mezei T,Pávai Z||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (48:95)||2007|
Lymphoma-like lesion of the uterine cervix: report of 12 cases of a rare entity.
MA5-11757 was used in immunohistochemistry to investigate the clinicopathologic features of 12 clinical cases of lymphoma-like lesions of the uterine cervix
|Ma J,Shi QL,Zhou XJ,Meng K,Chen JY,Huang WB||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (26:194)||2007|
Expression of Bcl-2 and Ki-67 in tamoxifen-associated endometrial polyps: comparison with postmenopausal polyps.
MA5-11757 was used in immunohistochemistry to compare the expression of Bcl-2 and ki-67 in tamoxifen-associated endometrial polyps and post-menopausal polyps
|Altaner S,Gucer F,Tokatli F,Guresci S,Ozdemir C,Puyan FO,Kutlu K||Onkologie (29:376)||2006|
Expression of caspase-3, p53 and Bcl-2 in generalized aggressive periodontitis.
MA5-11757 was used in immunohistochemistry to study the expression of caspase-3, p53 and Bcl-2 in generalized aggressive periodontitis
|Bulut S,Uslu H,Ozdemir BH,Bulut OE||Head & face medicine (2:null)||2006|
Bcl-2 expression predicts radiotherapy failure in laryngeal cancer.
MA5-11757 was used in immunohistochemistry to study the value of Bcl-2 expression in predicting the response to radiotherapy of laryngeal cancer patients
|Nix P,Cawkwell L,Patmore H,Greenman J,Stafford N||British journal of cancer (92:2185)||2005|
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
MA5-11757 was used in immunohistochemistry to investigate the influence of Bcl-2 reduction on the progression and prognosis of human IgA nephropathy
|Qiu LQ,Sinniah R,I-Hong Hsu S||Journal of the American Society of Nephrology : JASN (15:79)||2004|
Inhibition of proliferation and induction of autophagy by atorvastatin in PC3 prostate cancer cells correlate with downregulation of Bcl2 and upregulation of miR-182 and p21.
MA5-11757 was used in western blot to study the involvement of Bcl-2, miRNA-182 and p21 in the mechanism by which atorvastatin induces autophagy and inhibits the growth of prostate cancer cells
|Peng X,Li W,Yuan L,Mehta RG,Kopelovich L,McCormick DL||PloS one (8:null)||2013|
miR-129 promotes apoptosis and enhances chemosensitivity to 5-fluorouracil in colorectal cancer.
MA5-11757 was used in western blot to study the potential tumor suppressor activity of miRNA-129 and its role in promoting 5-fluorouracil sensitivity in colorectal cancer
|Karaayvaz M,Zhai H,Ju J||Cell death & disease (4:null)||2013|
Prohibitin induces apoptosis in BGC823 gastric cancer cells through the mitochondrial pathway.
MA5-11757 was used in western blot to study the role of the mitochondrial pathway in the mechanism by which prohibitin induces gastric cancer cell apoptosis
|Zhang L,Ji Q,Ni ZH,Sun J||Asian Pacific journal of cancer prevention : APJCP (13:3803)||2012|
Effect of different durations of overdistention on rat bladder function and morphology.
MA5-11757 was used in western blot to study changes in urinary bladder function and morphology following different periods of overdistension and the molecular mechanisms involved
|Jin XD,Cao M,Zhou XL,Yin HP,Chen ZD,Xu N,Jiang H||Brazilian journal of medical and biological research = Revista brasileira de pesquisas me¿dicas e biolo¿gicas / Sociedade Brasileira de Biofi¿sica ... [et al.] (45:921)||2012|
Cyclooxygenase-2 induces genomic instability, BCL2 expression, doxorubicin resistance, and altered cancer-initiating cell phenotype in MCF7 breast cancer cells.
MA5-11757 was used in western blot to examine the role of cyclooxygenase-2 in breast cancer sensitivity towards treatment
|Singh B,Cook KR,Vincent L,Hall CS,Berry JA,Multani AS,Lucci A||The Journal of surgical research (147:240)||2008|
Irreversible pan-ErbB tyrosine kinase inhibitor CI-1033 induces caspase-independent apoptosis in colorectal cancer DiFi cell line.
MA5-11757 was used in western blot to investigate the effect of pan-ErbB tyrosine kinase inhibitor CI-1033 on colorectal cancer cell apoptosis
|Skvortsov S,Skvortsova I,Sarg B,Loeffler-Ragg J,Lindner H,Lukas P,Tabernero J,Zwierzina H||Apoptosis : an international journal on programmed cell death (10:1175)||2005|
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; BCL2; protein phosphatase 1, regulatory subunit 50
AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50