|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues 61-76 of mouse bcl-2.|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bcl2 family is a key regulator of apoptosis that functions to either inhibit or promote cell death. Over-expression of members such as Bcl2 and BclxL inhibit the apoptotic process. The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protective effects of trimetazidine on bone marrow mesenchymal stem cells viability in an ex vivo model of hypoxia and in vivo model of locally myocardial ischemia.
MA1-12246 was used in western blot to study the effect of trimetazidine on the survival of bone marrow mesenchymal stem cells in a model of hypoxia and following transplantation into rat heart
|Xu H,Zhu G,Tian Y||Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (32:36)||2012|
Prenatal stress induces long-term effects in cell turnover in the hippocampus-hypothalamus-pituitary axis in adult male rats.
MA1-12246 was used in western blot to study the long-term effects of sub-chronic prenatal stress on cell turnover in the hippocampus-hypothalamus-pituitary axis in adult male rats
|Baquedano E,García-Cáceres C,Diz-Chaves Y,Lagunas N,Calmarza-Font I,Azcoitia I,Garcia-Segura LM,Argente J,Chowen JA,Frago LM||PloS one (6:null)||2011|
MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3.
MA1-12246 was used in western blot to study the mechanism by which the MyoD regulates myoblast apoptosis
|Hirai H,Verma M,Watanabe S,Tastad C,Asakura Y,Asakura A||The Journal of cell biology (191:347)||2010|
Growth hormone-releasing peptide 6 protection of hypothalamic neurons from glutamate excitotoxicity is caspase independent and not mediated by insulin-like growth factor I.
MA1-12246 was used in western blot to study the mechanism by which growth hormone-releasing peptide-6 protects a hypothalamic neuronal cell line against monosodium glutamate-induced apoptosis
|Delgado-Rubín A,Chowen JA,Argente J,Frago LM||The European journal of neuroscience (29:2115)||2009|
Anti-ceramidase LCL385 acutely reduces BCL-2 expression in the hippocampus but is not associated with an increase of learned helplessness in rats.
MA1-12246 was used in western blot to study the relationship between hippocampal apopotosis and learned helplessness in vivo using an anti-ceramidase compound
|Nahas Z,Jiang Y,Zeidan YH,Bielawska A,Szulc Z,Devane L,Kalivas P,Hannun YA||Behavioural brain research (197:41)||2009|
|Not Applicable||Not Cited||
Inhibition of GSK3 differentially modulates NF-kappaB, CREB, AP-1 and beta-catenin signaling in hepatocytes, but fails to promote TNF-alpha-induced apoptosis.
MA1-12246 was used in western blot to elucidate whether glycogen synthase kinase-3 inhibition induces apoptosis in the presence of TNF-alpha in primary mouse hepatocytes
|Götschel F,Kern C,Lang S,Sparna T,Markmann C,Schwager J,McNelly S,von Weizsäcker F,Laufer S,Hecht A,Merfort I||Experimental cell research (314:1351)||2008|
TACC3 depletion sensitizes to paclitaxel-induced cell death and overrides p21WAF-mediated cell cycle arrest.
MA1-12246 was used in western blot to study the effects of TACC3 depletion on sensitivity to paclitaxel-induced cell death and p21WAF-mediated cell cycle arrest
|Schneider L,Essmann F,Kletke A,Rio P,Hanenberg H,Schulze-Osthoff K,Nürnberg B,Piekorz RP||Oncogene (27:116)||2008|
Growth hormone releasing peptide-6 acts as a survival factor in glutamate-induced excitotoxicity.
MA1-12246 was used in western blot to study the role of growth hormone releasing peptide-6 as a survival factor in glutamate-induced excitotoxicity
|Delgado-Rubín de Célix A,Chowen JA,Argente J,Frago LM||Journal of neurochemistry (99:839)||2006|
Bcl-2 protects against oxidative stress while inducing premature senescence.
MA1-12246 was used in western blot to study the role of Bcl-2 in protecting against oxidative stress while inducing premature senescence
|López-Diazguerrero NE,López-Araiza H,Conde-Perezprina JC,Bucio L,Cárdenas-Aguayo MC,Ventura JL,Covarrubias L,Gutiérrez-Ruíz MC,Zentella A,Königsberg M||Free radical biology and medicine (40:1161)||2006|
Bcl-2 is a key factor for cardiac fibroblast resistance to programmed cell death.
MA1-12246 was used in western blot to study the role of Bcl-2 in heart fibroblast resistance to apoptosis
|Mayorga M,Bahi N,Ballester M,Comella JX,Sanchis D||The Journal of biological chemistry (279:34882)||2004|
Intrinsic and extrinsic pathway signaling during neuronal apoptosis: lessons from the analysis of mutant mice.
MA1-12246 was used in western blot to study the role of intrinsic and extrinsic signaling pathways in neuronal apoptosis
|Putcha GV,Harris CA,Moulder KL,Easton RM,Thompson CB,Johnson EM||The Journal of cell biology (157:441)||2002|
Iptakalim protects PC12 cell against H2O2-induced oxidative injury via opening mitochondrial ATP-sensitive potassium channel.
MA1-12246 was used in immunocytochemistry to study the protection of PC12 cells against H2O2-induced oxidative injury by iptakalim-mediated opening of mitochondrial ATP-sensitive potassium channels
|Chai Y,Niu L,Sun XL,Ding JH,Hu G||Biochemical and biophysical research communications (350:307)||2006|
Characterization of the signal that directs Bcl-x(L), but not Bcl-2, to the mitochondrial outer membrane.
MA1-12246 was used in immunocytochemistry and western blot to determine the signal characteristics that directs Bcl-x(L) and not Bcl-2 to the mitochondrial outer membrane
|Kaufmann T,Schlipf S,Sanz J,Neubert K,Stein R,Borner C||The Journal of cell biology (160:53)||2003|
Microbial colonization drives lymphocyte accumulation and differentiation in the follicle-associated epithelium of Peyer's patches.
MA1-12246 was used in immunohistochemistry (frozen) to determine the spatial relationships of B cells, T cells, and dendritic cells in Peyer's patches during microbial colonization.
|Yamanaka T,Helgeland L,Farstad IN,Fukushima H,Midtvedt T,Brandtzaeg P||Journal of immunology (Baltimore, Md. : 1950) (170:816)||2003|