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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli (His-Bcl-2)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||1 µl|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
A suggested positive control for this product is HL-60 cells.
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Developmental expression of Bcl-2 protein in human cortex.
LF-MA0199 was used in ELISA to study the developmental expression of Bcl-2 protein in human cortex
|Jarskog LF,Gilmore JH||Brain research. Developmental brain research (119:225)||2000|
B-cell CLL/lymphoma 2; Bcl-2; protein phosphatase 1, regulatory subunit 50
Bcl-2; BCL2; PPP1R50