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Immunofluorescence analysis of BCL-2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled BCL-2 (8C8) Mouse Monoclonal Antibody (MA513666) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A Synthetic peptide, aa 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13666 targets BCL-2 alpha in FACS, IF, IHC (P), and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-13666 immunogen is a Synthetic peptide, aa 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein.
Expression of Bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors.
MA5-13666 was used in immunohistochemistry to study the diagnostic value of the immunohistochemical expression of apoptotic and anti-apoptotic markers in serous and mucinous ovarian tumors
|Ozer H,Yenicesu G,Arici S,Cetin M,Tuncer E,Cetin A||Diagnostic pathology (7:null)||2012|
Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.
MA5-13666 was used in immunohistochemistry to study the influence of the phenotype of apoptosis-associated proteins on the clinical significance of cathepsin D expression in non-small cell lung cancer
|Fan C,Lin X,Wang E||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (33:1045)||2012|
Effects of estrogen replacement therapy on apoptosis and vascular endothelial growth factor expression in ocular surface epithelial cells: An experimental study.
MA5-13666 was used in immunohistochemistry to study rat ocular surface apoptosis and VEGF expression following estrogen replacement therapy
|Ozcura F,Dündar SO,Cetin ED,Beder N,Dündar M||International journal of ophthalmology (5:64)||2012|
Recurrence of benign meningiomas: predictive value of proliferative index, BCL2, p53, hormonal receptors and HER2 expression.
MA5-13666 was used in immunohistochemistry to identify biomarkers that predict the recurrence of benign meningiomas
|Abdelzaher E,El-Gendi SM,Yehya A,Gowil AG||British journal of neurosurgery (25:707)||2011|
The expression of IgM is helpful in the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma.
MA5-13666 was used in immunohistochemistry to investigate the effect of IgM expressoin on the differentiation of primary cutaneous diffuse large B cell lymphoma and follicle center lymphoma
|Demirkesen C,Tüzüner N,Esen T,Lebe B,Ozkal S||Leukemia research (35:1269)||2011|
Radiotherapy in laryngeal carcinoma: can a panel of 13 markers predict response?
MA5-13666 was used in immunohistochemistry to study the value of 13 immunohistochemical biomarkers in predicting response to radiotherapy in laryngeal cancer
|Wildeman MA,Gibcus JH,Hauptmann M,Begg AC,van Velthuysen ML,Hoebers FJ,Mastik MF,Schuuring E,van der Wal JE,van den Brekel MW||The Laryngoscope (119:316)||2009|
Prognostic significance of cell proliferation and apoptosis-regulating proteins in Epstein-Barr virus positive and negative pediatric Hodgkin lymphoma.
MA5-13666 was used in immunohistochemistry to evaluate prognostic markers for Epstein-Barr virus positive and negative pediatric Hodgkin lymphoma
|Aktaş S,Kargi A,Olgun N,Diniz G,Erbay A,Vergin C||Lymphatic research and biology (5:175)||2007|
Incidental calcifying fibrous tumor of the stomach presenting as a polyp.
MA5-13666 was used in immunohistochemistry to report on a case of incidental calcifying fibrous tumor of the stomach presenting as a polyp
|Elpek GO,Küpesiz GY,Ogüs M||Pathology international (56:227)||2006|
Prognostic factors in transitional cell cancer of the bladder: an emerging role for Bcl-2 and p53.
MA5-13666 was used in immunohistochemistry to study the prognostic value of Bcl-2 and p53 in transitional cell cancer of the bladder
|Ong F,Moonen LM,Gallee MP,ten Bosch C,Zerp SF,Hart AA,Bartelink H,Verheij M||Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology (61:169)||2001|
Curcumin-induced apoptosis in human leukemia cell HL-60 is associated with inhibition of telomerase activity.
MA5-13666 was used in western blot to study the potential role of telomerase inhibition in the pro-apoptotic effects of curcumin in a human leukemic cell line
|Mukherjee Nee Chakraborty S,Ghosh U,Bhattacharyya NP,Bhattacharya RK,Dey S,Roy M||Molecular and cellular biochemistry (297:31)||2007|
Tea-induced apoptosis in human leukemia K562 cells as assessed by comet formation.
MA5-13666 was used in western blot to study tea-induced apoptosis in human leukemia K562 cells
|Chakraborty S,Kundu T,Dey S,Bhattacharya RK,Siddiqi M,Roy M||Asian Pacific journal of cancer prevention : APJCP (7:201)||2006|
Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin.
MA5-13666 was used in western blot to study the mechanism of apoptosis induction in leukemic cells by the black tea polyphenol theaflavin
|Kundu T,Dey S,Roy M,Siddiqi M,Bhattacharya RK||Cancer letters (230:111)||2005|
B-cell CLL/lymphoma 2; BCL-2 alpha isoform; bcl-2-like protein 5; bcl-2-related protein A1; bcl2-L-5; hematopoietic BCL2-related protein A1; hemopoietic-specific early response protein; protein BFL-1; protein phosphatase 1, regulatory subunit 50
Bcl-2; BCL2; PPP1R50